Jie:LabNotes/oligo sequences
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20080716
primer to produce spacer:refer to LabNotes on [1]
CirclehelperV4_short(20080825):
AACAGTGCTCTTCCAGTCTACTAGCCTCATGCGTATCCGATC
20080904
Prmer used to amplify the cpg30k and cosmic3760. The amplicon will have 5-terminal phosphate, thus can be ligated after circlehelper insertion. AmpFV6.2_2: 5Phos/C*T*GCAGATGTTATCGAGGTC AmpRV6.2_2: 5Phos/CTGAATAGGAACGATGAGCC
20080918
confirmation primers for cpg30k. Primers targeting bisulfite conversed DNA to confirm the capture results of cpg30K. Media: 20080918_conirmed primers for cpg30k.xls
20080929
sapcer with length of 60mer that can be ligated without extension. Spacer_60mer_reverse: gacctcgataacatctgcagTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTGAGAATCCTTCCAGCTGAATAGGAACGATGAGCC Spacer_60mer: 5’-Phos/ctggaaggattctcaccaaacgcaacggtgattgtactggttctggtgttctactttgca Media:20080929_spacer of 60mer.doc
20081019
confirmation primers for cpg30k from cpg28k library. Media: 20081019_conirmed primers for cpg30k from cpg28k.xls
20081019
positive control used for cosmic probe circlehelper insertion. cosmic_probe_control: GTAGACTGGAAGAGCACTGTTGTTGGAGGCTCATCGTTCCTATTCAGCTGCAGATGTTATCGAGGTCCGACGATCGGATACGCATGAGGCTA
20081024
more confirmation primers for cpg30k from cpg28k library. Media: 20081024_confirmed primers plate_2 for cpg30k from cpg28k.xls Media: 20081024_conirmed primers plate_3 for cpg30k from cpg28k.xls