Kun:LabNotes/ASE/2007-10-30
Jump to navigation
Jump to search
Exp.1: Repeat PGP1Ver2 PCR on PGP1 gDNAs and Hues6 g/cDNA using NEB's 2X Taq Master mix
Set up reactions on ice:
One 33x Template 1ul 33ul 2X Taq Mix 25ul 825ul 50X SYBG I 0.4ul 13.2ul 10uM Primers 1ul each H2O 22.6ul 746ul
94C 2min -> 40 cycles of (94C 30S -> 57C 30S -> 68C 1min) -> 68C 3min on Chromo4
File:2007-10-31-PGP1VER2-PCR-plate2.jpg