Kun:LabNotes/ASE/2007-11-3

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Exp. 1 Perform PCR on the eSNP84k set 1-4 using iTaq.[edit]

        template:       0.2ul         1.6ul x 4 x 2
        10x buffer:      10ul          80ul x 4 x 2
        10mM dNTP:        2ul          16ul x 4 x 2
        100uMAP1V41U:   0.4ul         3.2ul x 4 x 2
        100uMphosAP2V4: 0.4ul         3.2ul x 4 x 2
        Taq:            0.5 or 1ul      8ul x 4 x 2
        50x SYBR I:     0.8ul         6.4ul x 4 x 2
        H2O:             86ul         680ul x 4 x 2

I set up 800ul reactions for each library first, but then wasn't quite confident that I add the round amount of enzyme and primers to each tube, so decided to do another 800ul.

94C 3min -> 17 cycles of (94C 45sec -> 58C 2min -> 72C 1min) -> 72C 3min -> 15C hold.