Kun:LabNotes/ASE/2007-11-6

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Exp. 1 PAGE purification of the eSNP84k probes[edit]

     Lib #1:  10ng/ul(380nM) x 40ul 
     Lib #2:  15ng/ul(580nM) x 40ul
     Lib #3:   5ng/ul(200nM) x 40ul
     Lib #4:  13ng/ul(500nM) x 40ul
     Lib #5:  12ng/ul(460nM) x 40ul

Exp. 2 Continue on the second round of probe prep.[edit]

Starting from the ethanol precipitated PCR amplicons from 800ul reactions.

The DNAs were resuspended in 100ul H2O. I added 10ul 10X NEBuffer 4, 4ul BciVI, 37C 3h, 75C 10min. I have one extra tube of Lib #5, which was included in the experiment.

Ethanol precipitation, resuspended in 45ul dH2O, added 5ul 10X lambda exo buffer, 3ul lambda exo, 37C 3h, 75C 10min, add 5ul USER, 37C overnight.