Kun:LabNotes/ASE/2007-11-6
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Exp. 1 PAGE purification of the eSNP84k probes[edit]
Lib #1: 10ng/ul(380nM) x 40ul Lib #2: 15ng/ul(580nM) x 40ul Lib #3: 5ng/ul(200nM) x 40ul Lib #4: 13ng/ul(500nM) x 40ul Lib #5: 12ng/ul(460nM) x 40ul
Exp. 2 Continue on the second round of probe prep.[edit]
Starting from the ethanol precipitated PCR amplicons from 800ul reactions.
The DNAs were resuspended in 100ul H2O. I added 10ul 10X NEBuffer 4, 4ul BciVI, 37C 3h, 75C 10min. I have one extra tube of Lib #5, which was included in the experiment.
Ethanol precipitation, resuspended in 45ul dH2O, added 5ul 10X lambda exo buffer, 3ul lambda exo, 37C 3h, 75C 10min, add 5ul USER, 37C overnight.