Kun:LabNotes/ASE/2007-12-14
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Exp.1 PCR on the circularized CES22k probes[edit]
one reaction 12 reactions Template: 10ul each 10x Buffer: 9ul 108ul 25mM MgCl2 5.4ul 64.8ul 25mM dNTP 1.2ul 13.2ul 100uM AmpSolV6F2 0.4ul 4.8ul 100uM AmpSolV6R2 0.4ul 4.8ul JumpStart Taq 1ul 12ul 50X SYBG I 0.8ul 9.6ul H2O 72ul 861ul
94C 3min -> 8 cycles of (94C 45sec -> 57C 1min -> 72C 1min) -> 10 cycles of (94C 45 sec -> 72 1min) -> 72C 3min -> 15C hold.
Took out 1C and 2A, add 4 more cycles on the remaining reactions.
File:2007-12-17-CES22k-SolLib-PAGE-quant.png
[12/15-12/17] PAGE purification: NA20431 2.48ng/ul 28nM GM20431 3.15ng/ul 36nM GC1F 2.05ng/ul 23nM GC1EP 0.58ng/ul 6.6nM Hues6g 2.69ng/ul 31nM Hues6c 2.53ng/ul 29nM H1g 3.09ng/ul 35nM H1c 1.41ng/ul 16nM H2g 4.08ng/ul 46nM H2c 0.68ng/ul 7.7nM
Repeat the PAGE quantification on 12/19/2007 H2g 5.48ng/ul H2c 0.46ng/ul BJc 2.08ng/ul
I got 118,945 reads from NA20431 in the Dec-19 run. The correlation of read number with probe abundance is R=0.145.