Kun:LabNotes/ASE/2007-12-20
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Exp.1 PCR on Hues6, GM20431, GC1F, GC1EP cDNA using the Hues6Ver2 primer set[edit]
The primer plate I ordered came in today. I made 10uM working primer mix by adding 5ul of forward and reverse primers into 90ul of ddH2O.
Template:
- Hues6 cDNA (04/18/07) 107ng/ul
- GM20431 dsCDNA (05/22/07) 62ng/ul
- GC1F sscDNA (06/05/07) 54ng/ul
- GC1EP sscDNA(06/15/07) 213ng/ul
Make dilution to 5ng/ul
Template: 1ul 2X Taq Mastermix: 15ul 2uM primers 3ul H2O: 11ul
94C 3min -> (94C 30sec -> 55C 30sec -> 72C 30sec) x 40 -> 72C 3min -> 4C hold.
File:2007-12-20-Hues6ver2-PCR.png