Kun:LabNotes/ASE/2008-1-15

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Exp.1 PCR on the eSNP84k capturing reactions started on 01/11/08[edit]

                          x 1         x 12
     template:           10ul         each
     JumpStart buffer:    9ul        108ul
     10mM dNTP:           2ul         24ul
     100uM AmpF2Sol:    0.4ul        4.8ul
     100uM AmpRSol:     0.4ul        4.8ul
     50X SYBG I:        0.8ul        9.6ul
     JumpStart:           2ul         24ul
     H2O:                75ul        900ul

94C 3min -> 8 cycles of (94C 45sec -> 56C 2min -> 72C 1min) -> 10 cycles of (94C 45 sec -> 72C 1min) -> 72C 3min -> 15C hold.

Exp.2 PCR on the circularized CES22k probes (01/13/08)[edit]

                              one reaction       4 reactions
          Template:             10ul                 each
          10x Buffer:            9ul                 36ul
          10mM dNTP              2ul                  8ul
          100uM AmpSolV6F2     0.4ul                1.6ul
          100uM AmpSolV6R2     0.4ul                1.6ul
          JumpStart Taq          2ul                  8ul
          50X SYBG I           0.8ul                3.2ul
          H2O                   72ul                288ul

94C 3min -> 8 cycles of (94C 45sec -> 58C 1min -> 72C 1min) -> 10 cycles of (94C 45 sec -> 72 1min) -> 72C 3min -> 15C hold.