Kun:LabNotes/ASE/2008-1-15
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Exp.1 PCR on the eSNP84k capturing reactions started on 01/11/08[edit]
x 1 x 12 template: 10ul each JumpStart buffer: 9ul 108ul 10mM dNTP: 2ul 24ul 100uM AmpF2Sol: 0.4ul 4.8ul 100uM AmpRSol: 0.4ul 4.8ul 50X SYBG I: 0.8ul 9.6ul JumpStart: 2ul 24ul H2O: 75ul 900ul
94C 3min -> 8 cycles of (94C 45sec -> 56C 2min -> 72C 1min) -> 10 cycles of (94C 45 sec -> 72C 1min) -> 72C 3min -> 15C hold.
Exp.2 PCR on the circularized CES22k probes (01/13/08)[edit]
one reaction 4 reactions Template: 10ul each 10x Buffer: 9ul 36ul 10mM dNTP 2ul 8ul 100uM AmpSolV6F2 0.4ul 1.6ul 100uM AmpSolV6R2 0.4ul 1.6ul JumpStart Taq 2ul 8ul 50X SYBG I 0.8ul 3.2ul H2O 72ul 288ul
94C 3min -> 8 cycles of (94C 45sec -> 58C 1min -> 72C 1min) -> 10 cycles of (94C 45 sec -> 72 1min) -> 72C 3min -> 15C hold.