Kun:LabNotes/ASE/2008-1-30
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Exp. 1 Prepare sequencing libraries for the CES27k captured SNPs[edit]
PAGE size selection of the 170bp band from all four PCR amplicons did on 01/29/08. The purified DNAs are resuspended in 20ul ddH2O.
Set up 2nd PCR.
DNA 20ul 2x Taq Master Mix 50ul 100uM SolAmpF6.2 0.4ul 100uM SolAmpR6.2 0.4ul 50X SYBG I 0.4ul H2O 30ul 94C 2min -> (94C 30sec -> 72C 1min) x 8 -> 72C 3min -> 15C hold. Purified with Qiaquick columns, measure concentration with Nanodrop and check with PAGE gel.