Kun:LabNotes/ASE/2008-6-27
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Sanger confirmation of ASE in PGP1[edit]
PCR[edit]
- Template:
- PGP1L: 137ng/ul, 06/03/08
- PGP1K: 80ng/ul, 06/03/08
- PGP1F: 101ng/ul, 06/24/08
- Primers: PGP1L-ASE-conf (06/25/08), 48 pairs.
x 50 x 3 Template: 4ng 2X TaqMaster mix: 15ul 750 5uM primer mix: 1.5ul each 50X SYBGI: 0.1ul 5 H2O 13ul 650 94C 2min -> 35x (94C 30sec -> 57C 30sec -> 72C 30sec) -> 72C hold. PGP1L/PGP1K File:2008-06-27-cDNA-PCR.png
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
A | X | X | X | X | X | X | X | X | X | X | X | O |
B | O | X | X | X | X | X | O | X | X | X | X | X |
C | X | X | X | X | X | X | X | X | X | X | X | X |
D | X | X | O | O | O | X | O | O | X | X | X | X |
E | X | O | O | X | X | X | O | O | X | X | X | X |
F | X | X | X | X | X | X | X | O | X | X | X | X |
G | O | X | O | X | O | X | X | X | X | X | O | X |
H | O | X | O | X | X | O | X | X | X | X | X | X |
PGP1F (A=====>H) File:2008-06-30-PGP1F-cDNA-PCR.png
- Also perform PCR on cDNA from PGP1L made on 12/31/07.
Layout of the plate shipped to Agencourt on 06/30/08 File:PGP1L-conf-submitted-sample-layout-06302008.png