Kun:LabNotes/CpgSeq/2007-11-14

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Exp.l PCR on the captured CpG islands[edit]

  • Template: two capturing reactions started at 11/12/2007.
  • Primer: Amp_F_sol, Amp_R_sol
                          x 1         x 2
     template:            5ul         10ul
     ABI 10x buffer:    4.5ul          9ul
     25mM MgCl2:          3ul          6ul
     10mM dNTP:           1ul          2ul
     100uM AmpFSol:    0.15ul        0.3ul
     100uM AmpRSol:    0.15ul        0.3ul
     50X SYBG I:        0.4ul        0.8ul
     iTaq:                1ul          2ul
     H2O:              35.3ul       70.6ul

94C 3min -> 10 cycles of (94C 45sec -> 59C 2min -> 72C 1min) -> 25 cycles of (94C 45 sec -> 72C 1min) -> 72C 3min -> 15C hold.