Kun:LabNotes/CpgSeq/2007-11-14
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Exp.l PCR on the captured CpG islands[edit]
- Template: two capturing reactions started at 11/12/2007.
- Primer: Amp_F_sol, Amp_R_sol
x 1 x 2 template: 5ul 10ul ABI 10x buffer: 4.5ul 9ul 25mM MgCl2: 3ul 6ul 10mM dNTP: 1ul 2ul 100uM AmpFSol: 0.15ul 0.3ul 100uM AmpRSol: 0.15ul 0.3ul 50X SYBG I: 0.4ul 0.8ul iTaq: 1ul 2ul H2O: 35.3ul 70.6ul
94C 3min -> 10 cycles of (94C 45sec -> 59C 2min -> 72C 1min) -> 25 cycles of (94C 45 sec -> 72C 1min) -> 72C 3min -> 15C hold.