Kun:LabNotes/CpgSeq/2007-12-14

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Exp.1 PCR on the circularized CpGMIPs probes[edit]

                              one reaction       8 reactions
          Template:             10ul                  each
          10x Buffer:            9ul                  80ul
          25mM dNTP            1.2ul                 9.6ul
          100uM AmpSolF        0.4ul                 3.2ul
          100uM AmpSolR        0.4ul                 3.2ul
          JumpStart Taq          1ul                   8ul
          50X SYBG I           0.8ul                 6.4ul
          H2O                   72ul                 576ul

94C 3min -> 8 cycles of (94C 45sec -> 58C 1min -> 72C 1min) -> 25 cycles of (94C 45 sec -> 72 1min) -> 72C 3min -> 15C hold.

 File:2007-12-14-CpgMIP-Capture-PCR.png

No band above 200bp was seen. Clearly no CpG island was captured.