Kun:LabNotes/CpgSeq/2007-12-14
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Exp.1 PCR on the circularized CpGMIPs probes[edit]
one reaction 8 reactions Template: 10ul each 10x Buffer: 9ul 80ul 25mM dNTP 1.2ul 9.6ul 100uM AmpSolF 0.4ul 3.2ul 100uM AmpSolR 0.4ul 3.2ul JumpStart Taq 1ul 8ul 50X SYBG I 0.8ul 6.4ul H2O 72ul 576ul
94C 3min -> 8 cycles of (94C 45sec -> 58C 1min -> 72C 1min) -> 25 cycles of (94C 45 sec -> 72 1min) -> 72C 3min -> 15C hold.
File:2007-12-14-CpgMIP-Capture-PCR.png
No band above 200bp was seen. Clearly no CpG island was captured.