Kun:LabNotes/CpgSeq/2007-12-30
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Exp.1 Perform PCR on the gel size selected fragment from the 12-23-07 PCR products[edit]
Into 100ul of eluted DNA, add: 25mM dNTP: 0.6ul 100uM AmpSolF: 0.2ul 100uM AmpSolR: 0.2ul JumpStart Taq: 0.5ul 50x SYBG I: 0.2ul
94C 3min -> (94C 30sec -> 72C 30sec) x 18 -> 72C 3min -> 4C hold.
Real-time Curves File:2007-12-30-CpG-PAGE-PCR.png File:2007-12-30-CpG-PAGE-PCR-gel.png
I don't think the CpGMIPs captured any sequences from the bisulfite converted genome. Perhaps there wasn't too much DNA? Or the gap is too long?