Kun:LabNotes/CpgSeq/2007-12-9
Jump to navigation
Jump to search
Exp.l PCR on the captured CpG islands[edit]
- Template: two capturing reactions started at 12/07/2007.
- Primer: Amp_F_sol, Amp_R_sol
x 1 x 4 template: 5ul JumpStart 10x buffer: 4.5ul 18ul 10mM dNTP: 1ul 4ul 100uM AmpFSol: 0.15ul 0.6ul 100uM AmpRSol: 0.15ul 0.6ul 50X SYBG I: 0.4ul 1.6ul JumpStart Taq: 0.5ul 2ul H2O: 35.3ul 142ul
94C 3min -> 10 cycles of (94C 45sec -> 58C 2min -> 72C 1min) -> 25 cycles of (94C 45 sec -> 70C 1min) -> 72C 3min -> 15C hold.
File:2007-12-09-CpG-MIP-PCR.png