Kun:LabNotes/CpgSeq/2007-12-9

From ZhangLabWiki
Jump to navigation Jump to search

Exp.l PCR on the captured CpG islands[edit]

  • Template: two capturing reactions started at 12/07/2007.
  • Primer: Amp_F_sol, Amp_R_sol
                              x 1         x 4
     template:                5ul         
     JumpStart 10x buffer:  4.5ul         18ul
     10mM dNTP:               1ul          4ul
     100uM AmpFSol:        0.15ul        0.6ul
     100uM AmpRSol:        0.15ul        0.6ul
     50X SYBG I:            0.4ul        1.6ul
     JumpStart Taq:         0.5ul          2ul
     H2O:                  35.3ul        142ul

94C 3min -> 10 cycles of (94C 45sec -> 58C 2min -> 72C 1min) -> 25 cycles of (94C 45 sec -> 70C 1min) -> 72C 3min -> 15C hold.

  File:2007-12-09-CpG-MIP-PCR.png