Kun:LabNotes/CpgSeq/2008-1-1
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Exp.1 Bisulfite conversion of DNA[edit]
Resuspend one vial of Bisulfite Mix in 800ul nuclease free water, votex for 5min. Set up reactions:
RL Jurkat DNA 10ul x 2 20ul x 2 Water 10ul x 2 Bisulfite mix 85ul x 2 85ul x 2 DNA Protection Buffer 35ul x 2 35ul x 2
Perform the bisulfite conversion on PTC-200 using the EpiTect program 99C5min->60C20min->99C5min->60C1h25min->99C5min->60C2h55min->20Chold.
Follow the EpiTect protocol to clean up the bisulphite converted DNAs.
Concentrate to 5ul with Speedvac.