Kun:LabNotes/CpgSeq/2008-1-13

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Exp.1 PCR on the CpGMIP capturing reaction.[edit]

                              one reaction       6 reactions
          Template:              5ul                  each
          10x Buffer:          4.5ul                  27ul
          25mM dNTP            0.6ul                 3.6ul
          100uM AmpSolF        0.2ul                 1.2ul
          100uM AmpSolR        0.2ul                 1.2ul
          JumpStart Taq          1ul                   6ul
          50X SYBG I           0.2ul                 1.2ul
          H2O                 38.8ul                 234ul

94C 3min -> 8 cycles of (94C 45sec -> 58C 1min -> 72C 1min) -> 10 cycles of(94C 30min -> 72C 1min)-> 72C 3min -> 15C hold.