Kun:LabNotes/CpgSeq/2008-1-13
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Exp.1 PCR on the CpGMIP capturing reaction.[edit]
one reaction 6 reactions Template: 5ul each 10x Buffer: 4.5ul 27ul 25mM dNTP 0.6ul 3.6ul 100uM AmpSolF 0.2ul 1.2ul 100uM AmpSolR 0.2ul 1.2ul JumpStart Taq 1ul 6ul 50X SYBG I 0.2ul 1.2ul H2O 38.8ul 234ul
94C 3min -> 8 cycles of (94C 45sec -> 58C 1min -> 72C 1min) -> 10 cycles of(94C 30min -> 72C 1min)-> 72C 3min -> 15C hold.