Kun:LabNotes/CpgSeq/2008-1-17

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PCR on the clonewell purified DNA[edit]

     Template:                 ~5ul
     2X iProof Master Mix:     25ul
     100uM AmpSolF:           0.2ul
     100uM AmpSolR:           0.2ul
     50X SYBG I:              0.2ul
     H2O:                    19.5ul
  98C 30sec -> 20 cycles of (98C 10sec -> 68C 30sec) -> 68C 1min -> 15CF hold