Kun:LabNotes/CpgSeq/2008-1-27
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Exp.1 Cpg capture with the second batch of probes[edit]
RL dsDNA(209ng/ul) RL ssDNA Template 6ul 8ul 10x buffer 1.8ul 1.8ul 104nM CpG28k 8ul 8ul H2O 2.2ul 0.2ul
95C 10min -> 60C 40h -> add 2ul NSL mix (Stoffel 2U/ul, AmpLigase 0.5U/ul, dNTP 50uM) -> 60C 1h - -> 37C 1min -> add 1ul Exo I&&III, 1ul T7 exo, 37C 2h -> 94C 5min -> 4C hold.