Kun:LabNotes/CpgSeq/2008-1-27

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Exp.1 Cpg capture with the second batch of probes[edit]

               RL dsDNA(209ng/ul) RL ssDNA  
    Template      6ul                8ul       
    10x buffer  1.8ul              1.8ul  
    104nM CpG28k  8ul                8ul 
    H2O         2.2ul              0.2ul

95C 10min -> 60C 40h -> add 2ul NSL mix (Stoffel 2U/ul, AmpLigase 0.5U/ul, dNTP 50uM) -> 60C 1h - -> 37C 1min -> add 1ul Exo I&&III, 1ul T7 exo, 37C 2h -> 94C 5min -> 4C hold.