Kun:LabNotes/CpgSeq/2008-1-28
Jump to navigation
Jump to search
Exp. 1 PCR on the CpG capturing reactions started on 01/26/2008[edit]
RL-ds JK-ds RL-ss JK-ss Template: 10ul 10ul 10ul 10ul 10X JumpStart buffer: 9ul 9ul 9ul 9ul 10mM dNTP 2ul 2ul 2ul 2ul 100uM AmpFV6.2Sol 0.4ul 0.4ul 0.4ul 0.4ul 100uM AmpRV6.2Sol 0.4ul 0.4ul 0.4ul 0.4ul 50X SYBG I 0.4ul 0.4ul 0.4ul 0.4ul JumpStart Taq 2ul 2ul 2ul 2ul H2O 76ul 76ul 76ul 76ul
94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 30sec) x 10 -> 72C 3min -> 4C hold.
Added 4 more cycles.
PAGE purification of the 400bp+, 300bp, 200bp bands.
File:2008-01-28-Cpg28k-capture-PCR.png