Kun:LabNotes/CpgSeq/2008-1-28

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Exp. 1 PCR on the CpG capturing reactions started on 01/26/2008[edit]

                          RL-ds    JK-ds      RL-ss      JK-ss   
    Template:              10ul     10ul       10ul       10ul   
    10X JumpStart buffer:   9ul      9ul        9ul        9ul  
    10mM dNTP               2ul      2ul        2ul        2ul  
    100uM AmpFV6.2Sol     0.4ul    0.4ul      0.4ul      0.4ul    
    100uM AmpRV6.2Sol     0.4ul    0.4ul      0.4ul      0.4ul
    50X SYBG I            0.4ul    0.4ul      0.4ul      0.4ul
    JumpStart Taq           2ul      2ul        2ul        2ul
    H2O                    76ul     76ul       76ul       76ul    

94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 30sec) x 10 -> 72C 3min -> 4C hold.

Added 4 more cycles.

PAGE purification of the 400bp+, 300bp, 200bp bands.

 File:2008-01-28-Cpg28k-capture-PCR.png