Kun:LabNotes/ExonomeSeq/2007-12-16

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Exp.1 Exon capture experiment[edit]

   NA10835(353ng/ul)        3ul
   E55kv1 probes 107nM     17ul
   10X Ampligase buffer  2.25ul

95C 10min -> 60C 40h -> add 2.5ul NSL mixv (1U/ul stoffel, 1U/ul AmpLigase, 200uM dNTP) -> 56C 1h -> (95C 2min -> 56C 1h) x 20 -> 37C 1min -> add 2ul exo I&III -> 37C 1h -> 95C 2min -> 4c hold