Kun:LabNotes/ExonomeSeq/2007-12-19

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Exp.1 PCR on the capturing reaction started on 12/16[edit]

    Template:              10ul
    10X JumpStart buffer:   9ul
    25mM dNTP               1ul
    100uM AmpFV6.2        0.4ul
    100uM AmpRV6.2        0.4ul
    50X SYBG I            0.4ul
    H2O                  78.8ul

94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 20 -> 72C 3min -> 4C hold.

 File:2007-12-19-E55kv1-capture-PCR.pngFile:2007-12-19-E55kv1-capture-PCR-size-selection.png

I decided to do gel purification to cut out the following bands:

  1. 150bp
  2. 280bp
  3. 350-400bp