Kun:LabNotes/ExonomeSeq/2007-12-19
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Exp.1 PCR on the capturing reaction started on 12/16[edit]
Template: 10ul 10X JumpStart buffer: 9ul 25mM dNTP 1ul 100uM AmpFV6.2 0.4ul 100uM AmpRV6.2 0.4ul 50X SYBG I 0.4ul H2O 78.8ul
94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 20 -> 72C 3min -> 4C hold.
File:2007-12-19-E55kv1-capture-PCR.pngFile:2007-12-19-E55kv1-capture-PCR-size-selection.png
I decided to do gel purification to cut out the following bands:
- 150bp
- 280bp
- 350-400bp