Kun:LabNotes/ExonomeSeq/2007-12-23
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Exp.1 PCR on the capturing reaction started on 12/21[edit]
Template: 10ul 10X JumpStart buffer: 9ul 25mM dNTP 1ul 100uM AmpFV6.2Sol 0.4ul 100uM AmpRV6.2Sol 0.4ul 50X SYBG I 0.4ul JumpStart Taq 1ul H2O 79ul
94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 1min) x 10 -> 72C 3min -> 4C hold.
File:2007-12-24-E55kv1-exon-capture-PCR.png
PAGE purification.
File:2007-12-27-E55kv1-PCR-PAGE.png