Kun:LabNotes/ExonomeSeq/2007-12-30
Jump to navigation
Jump to search
Exp. 1 Blunt-end cloning of the size selected PCR products from 12-23-07[edit]
Blunting of the DNA fragment[edit]
5X Phusion buffer: 1ul 10mM dNTP: 0.25ul DNA: 3.65ul Phusion: 0.1ul 72C 10min
Blunt-end ligation[edit]
pCR-Blunt 0.5ul Blunt PCR product 2ul 10X Ligation buffer 0.5ul Sterile water 1.5ul T4 DNA ligase (4U/ul) 0.5ul 16C 1h -> 65C 10min -> 4C hold.