Kun:LabNotes/ExonomeSeq/2008-1-17

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Exp.1 Perform 2nd round PCR on the PAGE purified 450bp band from the 12/27/07 experiment.[edit]

                         dNTP     dUTP
    Template:            5ul       5ul
    10X PCR buffer:     20ul      20ul
    10mM dNTP:           4ul       4ul
    1mM dUTP:            0ul       2ul
    100uM AmpFSolV6.2  0.8ul     0.8ul
    100uM AmpRSolV6.2  0.8ul     0.8ul
    50X SYBG I:        0.8ul     0.8ul
    JumpStart Taq:       4ul       4ul
    H2O:               160ul     158ul

94C 3min -> (94C 30sec -> 72C 1min) x 12cyc -> 72C 3min -> 15C hold.