Kun:LabNotes/ExonomeSeq/2008-1-22

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Exp. 1 2nd-round PCR on E55kv1 capturing amplicons[edit]

Templates:

  1. 1st-round amplicon(01/18/08)
  2. PAGE purified 500bp+ 1st-round amplicon(01/18/08)
                        #1         #2
   Template            10ul       10ul
   2X Taq Master mix  100ul      100ul
   100uM AmpFV6.2Sol  0.8ul      0.8ul
   100uM AmpRV6.2Sol  0.8ul      0.8ul
   50x SYBG I         0.8ul      0.8ul
   H2O                 89ul       89ul
   94C 2min -> (94C 30sec -> 72C 1min) x 10 -> 72C 3min -> 15C hold
  File:2008-01-22-E55kv1-PCR-2nd.png