Kun:LabNotes/ExonomeSeq/2008-1-27
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Exp.1 Perform exon capture experiment with the the second batch of Cosmic3760 probe set[edit]
NA10835(347ng/ul) 3ul NA20431(353ng/ul) 3ul Cosmid3760 probes 218nM (01/12/08,purified) 9ul 9ul 10X Ampligase buffer 1.5ul 1.5ul
95C 10min -> 60C 40h -> add 1.5ul NSL mix (Stoffel 2U/ul, AmpLigase 0.5U/ul, dNTP 50uM) -> 60C 1h -> 37C 1min -> add 1ul Exo I&&III, 1ul T7 exo, 37C 2h -> 94C 5min -> 4C hold.