Kun:LabNotes/ExonomeSeq/2008-1-27

From ZhangLabWiki
Jump to navigation Jump to search

Exp.1 Perform exon capture experiment with the the second batch of Cosmic3760 probe set[edit]

   NA10835(347ng/ul)                            3ul       NA20431(353ng/ul)  3ul
   Cosmid3760 probes 218nM (01/12/08,purified)  9ul                          9ul
   10X Ampligase buffer                        1.5ul                       1.5ul

95C 10min -> 60C 40h -> add 1.5ul NSL mix (Stoffel 2U/ul, AmpLigase 0.5U/ul, dNTP 50uM) -> 60C 1h -> 37C 1min -> add 1ul Exo I&&III, 1ul T7 exo, 37C 2h -> 94C 5min -> 4C hold.