Kun:LabNotes/ExonomeSeq/2008-1-28
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Exp. 1 PCR on the capturing reactions started on 01/26/2008[edit]
Cosmic3760 Template: 20ul 10X JumpStart buffer: 18ul 10mM dNTP 4ul 100uM AmpFV6.2Sol 0.8ul 100uM AmpRV6.2Sol 0.8ul 50X SYBG I 0.8ul JumpStart Taq 4ul H2O 152ul Split into two tubes.
94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 30sec) x 10 -> 72C 3min -> 4C hold.
I took one tube out after 18 cycles, and added 2 more cycles to the other tube.
File:2008-01-28-Cosmic3760-capture-PCR.png