Kun:LabNotes/ExonomeSeq/2008-1-28

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Exp. 1 PCR on the capturing reactions started on 01/26/2008[edit]

                          Cosmic3760     
    Template:              20ul        
    10X JumpStart buffer:  18ul       
    10mM dNTP               4ul        
    100uM AmpFV6.2Sol     0.8ul        
    100uM AmpRV6.2Sol     0.8ul         
    50X SYBG I            0.8ul        
    JumpStart Taq           4ul        
    H2O                   152ul          
    Split into two tubes.

94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 30sec) x 10 -> 72C 3min -> 4C hold.

I took one tube out after 18 cycles, and added 2 more cycles to the other tube.

   File:2008-01-28-Cosmic3760-capture-PCR.png