Kun:LabNotes/ExonomeSeq/2008-2-8

From ZhangLabWiki
Jump to navigation Jump to search

Sample submission for Solexa sequencing[edit]

Three libraries were submitted to Zhen Ye (Bing Ren's lab).

    Library                                Seq Primer
    Lib #1  GM10835PCR(column) 40nM   11ng/ul    SolSeqV6.3 (HPLC) 100uM
    Lib #2  GM20431PCR(PAGE)    5nM   21ng/ul    SolSeqV6.3 (HPLC) 100uM
    Lib #3  GM10835SolN6(PAGE) 40nM   3.4ng/ul   Sol1up  100uM

Data analysis[edit]

I got the Solexa sequencing reads back on Feb. 19, 2008.

          Samples               # raw reads     # mappable reads    #exonic targets
  Lane 3: GM10835PCR(column)    10,083,903        7,046,750           35,255
  Lane 4: GM20431PCR(PAGE)       5,449,192        4,806,792           33,092
  Lane 5: GM10835SolN6(PAGE)    11,607,310