Kun:LabNotes/ExonomeSeq/Overview 121207 020808

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Dec-12-2007 Probe prep for E55kv1 set
Dec-14-2007 PAGE purification of probes
Dec-16-2007 Exon capture with E55kv1 probes
Dec-17-2007 Another probe prep for E55kv1 set
Dec-19-2007 PCR on the captured exons (E55kv1); PAGE size selection
Dec-20-2007 PCR cloning
Dec-21-2007 Exon capture with E55kv1 probes
Dec-23-2007 Exp.1 PCR on the capturing reaction started on 12/21|PCR on captured exons (12/21/07); purified 200bp, 250bp, 350bp, 450bp bands
Dec-30-2007 Blunt-end cloning
Dec-31-2007 colony PCR
Jan-04-2008 colony PCR
Jan-06-2008 PAGE purification of E55kv1
Jan-08-2008 exon capture with the #P probe 2nd PCR on the PAGE purified 450bp band, make sequencing libs Probe prep for E55kv1 and CES22k#5
Jan-09-2008 submitted PCR amplicons to GeneWiz Sequencing lib construction (Mme I cut, PAGE purification)
Jan-13-2008 PCR on capture exon, did not work; Another round of exon capture with #P probes
Jan-14-2008 Sequencing lib construction (adding sequencing adaptors, PCR)
Jan-15-2008 PCR on captured exon, still did not work
Jan-16-2008 Another round of exon capture with #P and #NP probes
Jan-17-2008 2nd PCR on the PAGE purified 450bp band
Jan-18-2008 Sequencing library construction PCR on the captured exon, only the one with #NP probes worked
Jan-21-2008 Library construction (cont.) 2nd PCR on captured exon, and PAGE purified 500bp+ DNA
Jan-24-2008 Library construction (Mme I, USER/End repair/ligation or SolN6 synthesis; PCR) Probe prep for Cosmic 3760, CpG28k, CES27k sets
Jan-26-2008 Exon capture with Cosmic3760
Jan-27-2008 Another exon capture with Cosmic3760
Jan-28-2008 PCR on the 01/26/08 captured exons
Jan-29-2008 PCR on the 01/27/08 captured exons
Jan-30-2008 Library construction on the 01/29/08 amplicons
Feb-05-2008 CloneWell and PAGE purification
Feb-08-2008 Submitted 3 Solexa libs to Bing Ren's lab for sequencing