Dec-12-2007 |
Probe prep for E55kv1 set |
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Dec-14-2007 |
PAGE purification of probes |
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Dec-16-2007 |
Exon capture with E55kv1 probes |
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Dec-17-2007 |
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Another probe prep for E55kv1 set |
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Dec-19-2007 |
PCR on the captured exons (E55kv1); PAGE size selection |
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Dec-20-2007 |
PCR cloning |
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Dec-21-2007 |
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Exon capture with E55kv1 probes |
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Dec-23-2007 |
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Exp.1 PCR on the capturing reaction started on 12/21|PCR on captured exons (12/21/07); purified 200bp, 250bp, 350bp, 450bp bands |
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Dec-30-2007 |
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Blunt-end cloning |
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Dec-31-2007 |
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colony PCR |
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Jan-04-2008 |
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colony PCR |
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Jan-06-2008 |
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PAGE purification of E55kv1 |
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Jan-08-2008 |
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exon capture with the #P probe |
2nd PCR on the PAGE purified 450bp band, make sequencing libs |
Probe prep for E55kv1 and CES22k#5
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Jan-09-2008 |
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submitted PCR amplicons to GeneWiz |
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Sequencing lib construction (Mme I cut, PAGE purification) |
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Jan-13-2008 |
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PCR on capture exon, did not work; Another round of exon capture with #P probes |
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Jan-14-2008 |
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Sequencing lib construction (adding sequencing adaptors, PCR) |
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Jan-15-2008 |
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PCR on captured exon, still did not work |
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Jan-16-2008 |
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Another round of exon capture with #P and #NP probes |
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Jan-17-2008 |
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2nd PCR on the PAGE purified 450bp band |
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Jan-18-2008 |
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Sequencing library construction |
PCR on the captured exon, only the one with #NP probes worked |
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Jan-21-2008 |
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Library construction (cont.) |
2nd PCR on captured exon, and PAGE purified 500bp+ DNA |
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Jan-24-2008 |
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Library construction (Mme I, USER/End repair/ligation or SolN6 synthesis; PCR) |
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Probe prep for Cosmic 3760, CpG28k, CES27k sets
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Jan-26-2008 |
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Exon capture with Cosmic3760
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Jan-27-2008 |
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Another exon capture with Cosmic3760
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Jan-28-2008 |
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PCR on the 01/26/08 captured exons
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Jan-29-2008 |
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PCR on the 01/27/08 captured exons
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Jan-30-2008 |
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Library construction on the 01/29/08 amplicons
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Feb-05-2008 |
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CloneWell and PAGE purification
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Feb-08-2008 |
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Submitted 3 Solexa libs to Bing Ren's lab for sequencing
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