Matt:LabNotes/2013-3-12
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cDNA + RNase continued[edit]
- Used RNase A from Thermo Scientific
- Added 20ul H20 to each cDNA sample to bring total volume to 100ul
- Added 1.5ul RNase A (10ug/ul)
- Incubated for 15 min at 37C
- Purified cDNA with Qiagen minelute column
- Qubit conc:
Probe Capture[edit]