Matt:LabNotes/2013-5-16

From ZhangLabWiki
Jump to navigation Jump to search

Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-15

Column Purification[edit]

  • Purified 6 tubes of 200ul enzyme digested probes with 12 columns (100ul in each column)
  • Eluted 30ul each (180ul total per probe set)

Nanodrop ssDNA conc:
0 gap: 58.78 ng/ul * 180ul = 10.58ug
20 gap: 55.96 ng/ul * 180ul = 10.07ug

Continued on: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-17