Matt:LabNotes/2015-7-9
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DARTFISH Imaging Neurons[edit]
- Alex's iNGN Bipolar Neurons CA12kNov2014_suppv2
- Second Batch of Sebastian's Motor Neurons CA12kNov2014_suppv2
Universal Cy3 Dye-Probe check[edit]
- Add 75C pre-heated 0.5uM FISGA_Adpt (Cy3) (1ul 100uM FISGA_Adpt + 199ul 2X SSC + 30% formamide)
- Wash with 2X SSC twice and add 2X SSC for imaging
Alex's Bipolar Neurons[edit]
- ~30 rolonies per cell
File:Composite BipolarNeurons AdptCy3 Pos1.jpg File:Composite BipolarNeurons AdptCy3 Pos2.jpg
Sebastian's Motor Neurons[edit]
- ~80 rolonies per cell
File:Composite MotorNeurons AdptCy3 Pos1.jpg File:Composite MotorNeurons AdptCy3 Pos2.jpg