Matt:LabNotes/2015-9-25
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VECTABOND treat slides and coverslips[edit]
- Treated 50 22x22 #1.5 coverslips and 20 3x1in 1mm glass slides
Protocol[edit]
- Batches of 10 coverslips or 10 slides
- Add 7ml Vectabond reagent into 350ml acetone in glass jar and shake to mix, then put in 2 250ml beakers
- Immersed in acetone 250ml beaker for 5min
- Let extra acetone drip off and then immerse in Vectabond solution for 5min
- Dipped repeatedly in MilliQ water for 30 sec
- Air dried in 37C oven for 2 hours
Storage[edit]
- Coverslips stored in the coverslip racks in their cardboard boxes (10 each)
- Slides stored in original packaging