Matt:LabNotes/2015-9-25

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VECTABOND treat slides and coverslips[edit]

  • Treated 50 22x22 #1.5 coverslips and 20 3x1in 1mm glass slides

Protocol[edit]

  • Batches of 10 coverslips or 10 slides
  • Add 7ml Vectabond reagent into 350ml acetone in glass jar and shake to mix, then put in 2 250ml beakers
  1. Immersed in acetone 250ml beaker for 5min
  2. Let extra acetone drip off and then immerse in Vectabond solution for 5min
  3. Dipped repeatedly in MilliQ water for 30 sec
  4. Air dried in 37C oven for 2 hours

Storage[edit]

  • Coverslips stored in the coverslip racks in their cardboard boxes (10 each)
  • Slides stored in original packaging