Matt:LabNotes/qPCR Protocol
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qPCR with DNA standards[edit]
DNA Standards
- 10 ng/ul
- 1 ng/ul
- 0.1 ng/ul
- 0.01 ng/ul
Procedure: Standard qPCR
- 1 ul DNA template
- 15 ul 2x KAPA MasterMix
- 0.6 ul Forward primer
- 0.6 ul Reverse primer
- 12.8 ul H2O
Cycles of: 95C 0:10 58C 0:30 72C 2:00
Removed 2 tubes from each of the 4 samples every time one of their curves began to increase, suggesting that it reached levels differentiable from background noise. The DNA concentrations were measured