Noi/NOTES/2014-12-25
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Summary of WGBS sequencing libraries captured by SeqCap Hybridization capture and their corresponding index[edit]
1) HiSeq Rapid run at IGM (141216_SN1001_0423)[edit]
- Library type: WGBS libraries generated by KAPA Hyper Prep kit (all cancer plasma samples). NC samples were generated by original method but lacked T4 DNA Pol, T4 polynucleotide kinase.
- The WGBS libraries were captured by Nimblegen SeqCap LMS probe.
- PE 100+7
Library Name | Sample ID | Index# | Index Sequence | Adaptor/primer system | Sequencing lane |
NP-SeqCap-pCancerSetB-Dec4 TS#1 | PCP-2 | 1 | ATCACG | TruSeq | 1 |
NP-SeqCap-pCancerSetB-Dec4 TS#3 | PCP-4 | 3 | TTAGGC | TruSeq | 1 |
NP-SeqCap-pCancerSetB-Dec4 TS#8 | PCP-9 | 8 | ACTTGA | TruSeq | 1 |
NP-SeqCap-pCancerSetB-Dec4 TS#9 | 6P-1 | 9 | GATCAG | TruSeq | 1 |
NP-SeqCap-pCancerSetB-Dec4 TS#10 | 6P-2 | 10 | TAGCTT | TruSeq | 1 |
NP-SeqCap-pCancerSetB-Dec4 TS#11 | 6P-3 | 11 | GGCTAC | TruSeq | 1 |
NP-SeqCap-pCancerSetB-Dec4 TS#20 | 7P-3 | 20 | GTGGCC | TruSeq | 1 |
NP-SeqCap-pCancerSetB-Dec4 TS#21 | 7P-5 | 21 | GTTTCG | TruSeq | 1 |
NP-SeqCap-pCancerSetB-Dec4 TS#22 | 7P-6 | 22 | CGTACG | TruSeq | 1 |
NP-SeqCap-pCancerSetB-Dec4 TS#23 | 7P-7 | 23 | GAGTGG | TruSeq | 1 |
NP-SeqCap-pCancerSetB-Dec4 TS#25 | 7P-8 | 25 | ACTGAT | TruSeq | 1 |
NP-SeqCap-pCancerSetB-Dec4 TS#27 | 7P-10 | 27 | ATTCCT | TruSeq | 1 |
NP-SeqCap-pCancerSetB-Dec4 TS#7 | NC-7 | 7 | CAGATC | TruSeq | 1 |
NP-SeqCap-tCancerSetC-Dec4 N2#20 | 6T-1 | 4 | TGACCA | STD ILMN adaptor/PCR_R.N2 IndX | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2#21 | 6T-2 | 5 | ACAGTG | STD ILMN adaptor/PCR_R.N2 IndX | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2#22 | 6T-3 | 6 | GCCAAT | STD ILMN adaptor/PCR_R.N2 IndX | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2#23 | 6T-4 | 7 | CAGATC | STD ILMN adaptor/PCR_R.N2 IndX | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2#24 | 6T-5 | 8 | ACTTGA | STD ILMN adaptor/PCR_R.N2 IndX | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2#25 | 7T-1 | 9 | GATCAG | STD ILMN adaptor/PCR_R.N2 IndX | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2#26 | 7T-2 | 10 | TAGCTT | STD ILMN adaptor/PCR_R.N2 IndX | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2#28 | 7T-4 | 12 | CTTGTA | STD ILMN adaptor/PCR_R.N2 IndX | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2#29 | 7T-5 | 13 | CCATGA | STD ILMN adaptor/PCR_R.N2 IndX | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2#30 | PCT-1 | 14 | AAGACA | STD ILMN adaptor/PCR_R.N2 IndX | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2#31 | PCT-2 | 15 | CTTCCT | STD ILMN adaptor/PCR_R.N2 IndX | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2#32 | PCT-4 | 16 | GGGGTT | STD ILMN adaptor/PCR_R.N2 IndX | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2#33 | PCT-6 | 17 | CTCATC | STD ILMN adaptor/PCR_R.N2 IndX | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2#34 | PCT-7 | 18 | AGTTCA | STD ILMN adaptor/PCR_R.N2 IndX | 2 |
NP-SeqCap-tCancerSetC-Dec4 TS#6 | NC-6 | 6 | GCCAAT | TruSeq | 2 |