Noi/NOTES/2014-12-25

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Summary of WGBS sequencing libraries captured by SeqCap Hybridization capture and their corresponding index[edit]

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1) HiSeq Rapid run at IGM (141216_SN1001_0423)[edit]

  • Library type: WGBS libraries generated by KAPA Hyper Prep kit (all cancer plasma samples). NC samples were generated by original method but lacked T4 DNA Pol, T4 polynucleotide kinase.
  • The WGBS libraries were captured by Nimblegen SeqCap LMS probe.
  • PE 100+7
Library Name Sample ID Index# Index Sequence Adaptor/primer system Sequencing lane
NP-SeqCap-pCancerSetB-Dec4 TS#1 PCP-2 1 ATCACG TruSeq 1
NP-SeqCap-pCancerSetB-Dec4 TS#3 PCP-4 3 TTAGGC TruSeq 1
NP-SeqCap-pCancerSetB-Dec4 TS#8 PCP-9 8 ACTTGA TruSeq 1
NP-SeqCap-pCancerSetB-Dec4 TS#9 6P-1 9 GATCAG TruSeq 1
NP-SeqCap-pCancerSetB-Dec4 TS#10 6P-2 10 TAGCTT TruSeq 1
NP-SeqCap-pCancerSetB-Dec4 TS#11 6P-3 11 GGCTAC TruSeq 1
NP-SeqCap-pCancerSetB-Dec4 TS#20 7P-3 20 GTGGCC TruSeq 1
NP-SeqCap-pCancerSetB-Dec4 TS#21 7P-5 21 GTTTCG TruSeq 1
NP-SeqCap-pCancerSetB-Dec4 TS#22 7P-6 22 CGTACG TruSeq 1
NP-SeqCap-pCancerSetB-Dec4 TS#23 7P-7 23 GAGTGG TruSeq 1
NP-SeqCap-pCancerSetB-Dec4 TS#25 7P-8 25 ACTGAT TruSeq 1
NP-SeqCap-pCancerSetB-Dec4 TS#27 7P-10 27 ATTCCT TruSeq 1
NP-SeqCap-pCancerSetB-Dec4 TS#7 NC-7 7 CAGATC TruSeq 1
NP-SeqCap-tCancerSetC-Dec4 N2#20 6T-1 4 TGACCA STD ILMN adaptor/PCR_R.N2 IndX 2
NP-SeqCap-tCancerSetC-Dec4 N2#21 6T-2 5 ACAGTG STD ILMN adaptor/PCR_R.N2 IndX 2
NP-SeqCap-tCancerSetC-Dec4 N2#22 6T-3 6 GCCAAT STD ILMN adaptor/PCR_R.N2 IndX 2
NP-SeqCap-tCancerSetC-Dec4 N2#23 6T-4 7 CAGATC STD ILMN adaptor/PCR_R.N2 IndX 2
NP-SeqCap-tCancerSetC-Dec4 N2#24 6T-5 8 ACTTGA STD ILMN adaptor/PCR_R.N2 IndX 2
NP-SeqCap-tCancerSetC-Dec4 N2#25 7T-1 9 GATCAG STD ILMN adaptor/PCR_R.N2 IndX 2
NP-SeqCap-tCancerSetC-Dec4 N2#26 7T-2 10 TAGCTT STD ILMN adaptor/PCR_R.N2 IndX 2
NP-SeqCap-tCancerSetC-Dec4 N2#28 7T-4 12 CTTGTA STD ILMN adaptor/PCR_R.N2 IndX 2
NP-SeqCap-tCancerSetC-Dec4 N2#29 7T-5 13 CCATGA STD ILMN adaptor/PCR_R.N2 IndX 2
NP-SeqCap-tCancerSetC-Dec4 N2#30 PCT-1 14 AAGACA STD ILMN adaptor/PCR_R.N2 IndX 2
NP-SeqCap-tCancerSetC-Dec4 N2#31 PCT-2 15 CTTCCT STD ILMN adaptor/PCR_R.N2 IndX 2
NP-SeqCap-tCancerSetC-Dec4 N2#32 PCT-4 16 GGGGTT STD ILMN adaptor/PCR_R.N2 IndX 2
NP-SeqCap-tCancerSetC-Dec4 N2#33 PCT-6 17 CTCATC STD ILMN adaptor/PCR_R.N2 IndX 2
NP-SeqCap-tCancerSetC-Dec4 N2#34 PCT-7 18 AGTTCA STD ILMN adaptor/PCR_R.N2 IndX 2
NP-SeqCap-tCancerSetC-Dec4 TS#6 NC-6 6 GCCAAT TruSeq 2