Noi/NOTES/2014-6-3

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RRBS, tumor tissue sample (colon)[edit]

Link to calendar: [[1]]

  • Experimental procedur ewill be added

PAGE Quantification[edit]

  • Loaded 4ul of 1X bead-purified sequencing libraries (total 60ul) in 12% TBE gel
File:ZhangLab 2 2014-06-06 08hr 41min PQ 5ng-CTT-RRBS 4ul.jpg
Sample TruSeq Index Conc. (ng/ul) Yield in 60ul (ng) Amount for 150ng (ul)
CTT-frozen 5ng_1 Ind_9 6.66 399.60 22.52
CTT-frozen 5ng_2 Ind_10 15.04 902.62 9.97
CTT-FFPE 5ng_1 Ind_11 9.60 575.94 15.63
CTT-FFPE 5ng_2 Ind_13 15.09 905.13 9.94

PAGE size selection[edit]

  • Performed PAGE size selection in 3 wells of 5-well 12% TBE gel
File:ZhangLab 2 2014-06-06 10hr 08min 5ng-CTT-RRBS PAGE-SS.jpg
  • Verified 2ul of size-selected libraries in 6% TBE gel
File:ZhangLab 2 2014-06-07 22hr 50min PAGE-verify NP-RRBS-CTT-5ng.jpg

Sequencing library ID: NP-RRBS-CTT-5ng, Jun3, 2014

- Length: 160-360bp
- Index: Truseq Ind9-11, 13