Noi:Probe preparation data/Probes Prep

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Summary of DMR220k probe set production[edit]

Probe set No. of rxn Date of production Enzyme Conc. (ng/ul) Vol. (ul) Yields (ug) for DNA capture
S1 96 rxn 2010_10_24 Kapa 26.20 250.00 6.50 diluted to 2ng/ul vol 100ul
S2 96 rxn 2010_10_24 Kapa 30.10 250.00 7.50 diluted to 2ng/ul vol 100ul
S3 96 rxn 2010_10_24 Kapa 23.20 250.00 5.80
S4 96 rxn 2010_10_21 Kapa 24.80 200.00 4.90
S5 96 rxn 2010_10_21 Kapa 26.70 200.00 5.30
S6 96 rxn 2010_10_22 Kapa 27.70 200.00 5.50
S7 96 rxn 2010_10_22 Kapa 32.00 200.00 6.40
1st prep Total (ug)
S6 96 rxn 2010_10_01 EconoTaq 24.60 102.00 2.50 8.00
S7 96 rxn 2010_09_27 Kapa 36.90 200.00 7.30 13.70


Probe set Amplified amplicon yields (ug) ss-amplicon yields (ug) USER/DpnII digested DNA yields (ug) % digestion, %recovery PAGE size selection yields (ug)
S1 86.6 22.2 13 92%, 54% 6.5
S2 97 26.5 15.6 92%, 52% 7.5
S3 97.2 24.4 13.8 94%, 44% 5.8
S4 95.5 19.5 10.7 93%, 49% 4.9
S5 95.8 18.2 9.6 92%, 60% 5.3
S6 95.8 23.7 12.2 >90%, 51% 5.5
EconoTaq 58.5 8.7 5.1 2.5
S7 88.6 23.5 12.4 93%, 55% 6.4
95.4 21.2 11.8 7.3