Noi:Probe preparation data/Probes Prep
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Summary of DMR220k probe set production[edit]
Probe set | No. of rxn | Date of production | Enzyme | Conc. (ng/ul) | Vol. (ul) | Yields (ug) | for DNA capture |
S1 | 96 rxn | 2010_10_24 | Kapa | 26.20 | 250.00 | 6.50 | diluted to 2ng/ul vol 100ul |
S2 | 96 rxn | 2010_10_24 | Kapa | 30.10 | 250.00 | 7.50 | diluted to 2ng/ul vol 100ul |
S3 | 96 rxn | 2010_10_24 | Kapa | 23.20 | 250.00 | 5.80 | |
S4 | 96 rxn | 2010_10_21 | Kapa | 24.80 | 200.00 | 4.90 | |
S5 | 96 rxn | 2010_10_21 | Kapa | 26.70 | 200.00 | 5.30 | |
S6 | 96 rxn | 2010_10_22 | Kapa | 27.70 | 200.00 | 5.50 | |
S7 | 96 rxn | 2010_10_22 | Kapa | 32.00 | 200.00 | 6.40 | |
1st prep | Total (ug) | ||||||
S6 | 96 rxn | 2010_10_01 | EconoTaq | 24.60 | 102.00 | 2.50 | 8.00 |
S7 | 96 rxn | 2010_09_27 | Kapa | 36.90 | 200.00 | 7.30 | 13.70 |
Probe set | Amplified amplicon yields (ug) | ss-amplicon yields (ug) | USER/DpnII digested DNA yields (ug) | % digestion, %recovery | PAGE size selection yields (ug) |
S1 | 86.6 | 22.2 | 13 | 92%, 54% | 6.5 |
S2 | 97 | 26.5 | 15.6 | 92%, 52% | 7.5 |
S3 | 97.2 | 24.4 | 13.8 | 94%, 44% | 5.8 |
S4 | 95.5 | 19.5 | 10.7 | 93%, 49% | 4.9 |
S5 | 95.8 | 18.2 | 9.6 | 92%, 60% | 5.3 |
S6 | 95.8 | 23.7 | 12.2 | >90%, 51% | 5.5 |
EconoTaq | 58.5 | 8.7 | 5.1 | 2.5 | |
S7 | 88.6 | 23.5 | 12.4 | 93%, 55% | 6.4 |
95.4 | 21.2 | 11.8 | 7.3 |