Noi:Samples from Illumina
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- I will add previous samples from Xuyu (bisDNA and pre-amplified DNA for BSPP capture)
2014-08-12[edit]
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- Received ~5ul of cfDNA (only info. available on the sample tube) from Xuyu. She said the concentration is 16g/ul. I add more TE buffer to bring volume to 20l and re-quantified concentration by Qubit dsDNA HS assay. The concentration is 7.09ng/ul.
2014-08-25[edit]
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- Dr. Zhang received 3 additional cfDNA from Illumina
- Sample info. from Rigo
Sample
|
Conc. (ng/ul)
|
Volume (ul)
|
Amount (ng)
|
NORM1-02 P1 |
5.25 |
15.00 |
78.70
|
NORM1-03 P1 |
3.25 |
15.00 |
48.70
|
COLO2-03 |
3.05 |
15.00 |
45.80
|
- I am not sure how they measure DNA concentration. I will repeat measuring concentration again.
- To avoid losing too much DNA for re-quantification, I will add more ~7ul of EB buffer to bring the volume to 21ul and use 1ul for Qubit dsDNA quantification.
Re-quantification of cfDNA by Qubit dsDNA HS assay[edit]
Sample
|
Conc. By Qubit (ng/ul)
|
Amount in 20ul (ng)
|
NORM1-02 P1 |
1.66 |
33.20
|
NORM1-03 P1 |
0.77 |
15.30
|
COLO2-03 |
1.11 |
22.20
|
- I guess that the concentration of cfDNA from Rigo is by Nanodrop. Those numbers seemed to be over-estimated since the total amount by Qubit is much lower than that I got from Illumina.