Ns125:LabNotes/2015-8-4

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  • MBD3 worked --> large scale PCR & size selection. 120 ng template, 2x50 uL rxns
  • Test SOX2, FOXA2 conditions --> double template conc., 10 cycles w/ 70 C annealing temp, 25 sec extension time
  • Received ctrl gRNA --> clone as if library
  • MBD3 adapter PCR --> index 22, 2x50 uL rxns w/ 25 ng template each
  • Dilute library for sequencing