Ns125:LabNotes/2016-4-5

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  • DropSeq beads purification --> cDNA concentrations low (6 out of 8 < 0.05 ng/uL)
  • 23 cycles gRNA PCR --> no bands on gel
  • Use remaining 1600 beads to repeat SMART PCR
  • DropSeq beads purification, 30 uL for cDNA, 10 uL for gRNA
  • cDNA concentration still low --> something wrong with beads or RT