Rui:10.10.13 discussion

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10.10.13 discussion[edit]

  • Current methods
			Efficiency	Ref(10pg)	SC(mNPC,mES)	SN(nuclei)
Smart-seq		10%		+		+		N/A
Smart-seq2		40% (est.)	
ILMN-dT/N6-9		?		+		+(?)

C1/Smart-seq		10-20%				+		+(?)
STRT/C1 (Stenn, UMI)	48%	
Cel-seq		17%
  • Methods plan
# STRT/C1 integrated with frag./PAP
# Cel-seq integrated with frag./PAP
# fine tune PAP: control length
# different T20/T30 primers: SEvsPE, T20/T30, VN-V-none
  • Upstream
# cell sorting
# C1 fluidigm
# LCM
  • Downstream
# data analysis: TSCC, Kun's scripts
# Wei's group