Rui:Bio-Rad QPCR machine

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Bio-Rad QPCR machine[edit]

  1. Program on desktop: "Opticon Moniter 3"
  2. Instrument: Chromo4:CD003042. "Instrument"-->"Scan for instruments" or "Quick load"(under "Master")-->choose "Chromo4:CD003042"
  3. "Prepare new run" or "open" a old file and "edit"
  4. "Plate setup"-->"edit"-->select "samples" and "SBG1" for SYBR green I
  5. "Protocol setup"-->"edit"-->add "plate read" before "repeat cycle"
  6. "Melting curve" optional, hold at 15C, volume 20ul
  7. "Run" and "Save", files are saved in "Opticon Users 3"/Rui
  8. "Status" and "Quantitation" are used for monitoring PCR process
  9. "Quantitation"-->"copy to clipboard"-->"Data graph" and edit under "Paint"