Rui:Ep Realplex QPCR machine
Jump to navigation
Jump to search
Ep Realplex QPCR machine[edit]
- "Mastercycler ep realplex" on desktop
- log in User name: "EPPENDORF", password: "e"
- "PCR program"-->direct change temp and time-->right click to insert and advance edit
- "Plate layout"-->"Filer 520nm"-->"SYBR"; "Sample volume", "Probe"-->"SYBR green", "Background"-->"Biorad strip tube-50ul" (Attention!); select sample area--> right click-->"unknown"-->ID
- "Save as" a "template" or an "assay"
- "Set up"-->"System Configuration"-->"Cycler"-->"Lid"-->select "Heat"-->Okey and close for preheat the lid
- "Monitoring" for PCR process
- "Analysis data" and save as assay