Rui:Ep Realplex QPCR machine

From ZhangLabWiki
Jump to navigation Jump to search

Ep Realplex QPCR machine[edit]

  1. "Mastercycler ep realplex" on desktop
  2. log in User name: "EPPENDORF", password: "e"
  3. "PCR program"-->direct change temp and time-->right click to insert and advance edit
  4. "Plate layout"-->"Filer 520nm"-->"SYBR"; "Sample volume", "Probe"-->"SYBR green", "Background"-->"Biorad strip tube-50ul" (Attention!); select sample area--> right click-->"unknown"-->ID
  5. "Save as" a "template" or an "assay"
  6. "Set up"-->"System Configuration"-->"Cycler"-->"Lid"-->select "Heat"-->Okey and close for preheat the lid
  7. "Monitoring" for PCR process
  8. "Analysis data" and save as assay