Rui:LabNotes/SingleCell/2011-1-20

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1. Measure the concentration of the RNA samples[edit]

--------------------------------------------------------------
RNA ID          [ng/ul]        260/280         260/230
-------------------------------------------------------------- 
Hues54          414.50	        2.06	        2.01
Hues6-ES        641.13	        2.12	        2.11
Hues6-EB        561.99	        2.11	        2.11
--------------------------------------------------------------

2. Examine the integrity of the RNA samples[edit]

  • 25ml agarose gel + 2ul SYBR safe

File:ZhangLab 2 2011-01-20 11hr 50min.jpg

3. Dilute RNA samples in PCR 8-tube strip (-80C)[edit]

  • 1) 1:10
  • 2) 1:10
  • 3) 1:4/1:6/1:5.6 --> 1ng/ul
  • 4) 1:5 --> 0.2ng/ul
  • 5) 1:10 --> 0.02ng/ul