Rui:LabNotes/SingleCell/2011-5-17
Sequence result[edit]
Continue with lab note on 5.16.11
RNA sample Hues54[edit]
- 15 colonies directly for TempliPhi, roughly ~500ng/ul in 10ul PCR, 3ul sending for sequencing (M13R provided by Genewiz)
- No priming: 3
- mRNA: 11
- Potential non-coding region: 1
RNA-1: poor quality; first 60bp match to intergenic region with 88%
>RNA-1-M13R_F01.ab1
NNNNNNANNNTGNNNNNNGCTCGGATCCNCTAGTAACGGCCGCCNGTGTGCTGGAATTCGGCTTTAATACGACTCACNNN
NGGNGTATGCTTTTTTTTTTTTTTTTTTTTTTTTTCCNCNGGAANAANTGCACCTGTGTCGCCNTCCCACAGGGGAACTC
GGGAGACAAAGGCGCTTTGNCNCNTCTGGGGGANCCTATCAGGACCAATTCCCATGGCGAATACCATGATATCGTCCGTN
ACTGGGGGGANANTGACNTGGNTCCNAANACTGNGGNTCGGCCCGATNTTTANAAAAAAACNAACGCTAAANAAAATTGA
CAATTCCAAATTAACCGGTTCCCCGTAGANCTTTCGGGGGNGGGGANCNGGGGANNNNNNNGGNCTTTCCNNCNNCCANA
ANAAAAAAGAAAGGGACGGGCCGGGGAAAGGCCGGNGAAACAGGCNATAGNNCCTAGGACANTGNNNATGATNAAGACCG
ACTAATGGGGGCTGCGGNCCCGTGTGTANGNGTCAGGCTGTTTCTTTTGGNANGAAGAGGGGTGGTCTTTCATANAATTC
GGTGGGGGGTGGGCTCGGAGAGGGTCTACTTTGTGGAGAGNATGANGGCCAANNNANNCCNAAAGCCNAGCACNTCGGCG
AAGATCAGAATCCGANNTACCNNTN
RNA-2: "poor quality"; spliced form of RPL35, 98.1% >RNA-2-M13R_F02.ab1 NNNNNNNNNNNNNNNNNNNNNCTCGGANCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCACT ATAGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTGGAGCTGCTGAAACAGCTGGACGACCTGAAGGTGGAGCTGTCCN NNCTGCGCGTCGCCAAAGTGACAGGGGGNGCGGCCTCCAAGCTCTCTAAGATCCGAGTCGTCCGGAAATCCATTGCCCGT GTTCTCACAGTTATTAACCAGACTCAGAAAGAAAACCTCAGGAAATTCTACAAGGGCAAGTAGTACAAGCCCCTGGACCT GCGGCCTAAGAAGAAACGTGCCATGCNCCGCCGGCTCAATAANCACGANGANAACCTGAAAACCAAGAAGCAGCAGCGGA AGGAGCGGCTGTACCCGCTGCGGAAATACCCGGTCAAGGNCTGAGGGGCGCATTGTCAATAAAGCACAGCTGGNTGAGAA A
RNA-3: "poor quality; spliced form of BQBP1, 98.4% >RNA-3-M13R_F03.ab1 NNNNNNNNNNNNNNNNNNNNNNCTNNNNATCCNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCA CTNNNGGGGGANCCTTTTTTTTTTTTTTTTTTTTTTTTTCCCNNGANANGNTCTGNCAGGGATCGAGAGCGTGGNTATGA CAAGGTAGACAGANAGAGAGAGCGANNGNNNNAACGGGATCGGGACCGCGGGTATGANAAGGCAGACCGGGAANAGGGCA AAGAAGGGCGCCCCCATCGCCGGGAGGAGCNGNCTCCCTATCCCAAGAGCNAGAAGGCAGTAAGCCGAAAGGATGAAGAG TTANACCCCNTGGACCCTAGCTCATACNCNNACGCCCCCCGGGGCNGTNGNCAACAGGANTCCCCAAGNGGAATGAGGCN AAAAN
RNA-4: PQ; MTF1, 98.6% >RNA-4-M13R_F04.ab1 NNNNNNNNNNNNNNNNNNNNNTCGGATCCNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCACTA NAGGGGNNNCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGCCGACAAGCAGAGACTCCTTCAGACCCTCAAAC AGAAACATTAAGTGCCATGGATGTGTNAGAGTTTCTNTCCCTCCAGAGCCTGGACACCCCGTCCAATCTGATTCCCATTG AAGCACTACTGCAGGGGGAGGAGGAGATGGGCCTCACCAGCAGCTTCTCCAAGTNAAGGGCCCATGTGTGCTCCCCTCTG GGAAAAGCGGGTGAGCAGGAGGCATGAGGTACAATGCCTGCCATCATGGGTCAAAAATTTGAAGGATGAAAAAATCTACT GTTTGAAATCCTCACCTTTTAGACGTATTTTTTTTATTCAN
RNA-5: PQ; SNX9, 99.5% >RNA-5-M13R_F05.ab1 NNNNNNNNNNNNNNNNNCNNANNNNNNNNNCACNANTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCAC TATANNGNGNGNNTTTTTTTTTTTTTTTTTTTTTTTAACAGGNNCNNGNNACACCGTCAGCTCGAACTCAACACTGGCAG CCACCGTCTCACCCCTGCGGAGGAGCGCTCCCGTCTCCCACAGGTGCCTTACCGCGTTCCCTCCCGCTGCTTTCATTTTT CTGACCTAATAATTACGGGAAANGGAAAGTCTGGGCCANCANCNATAAAATGACACCAAAAATAAGTANANGANATCGAA T
RNA-6: no priming
RNA-7:PQ; CR594125, 94.6% >RNA-7-M13R_F07.ab1 NNNNNNNNNNNNTGNNNNNAGCTCGNATNNNNTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCACT ANNGGGGNNNCCNTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTCCCCGTGGTTTAATATGAACAGAGTTGAATATGACAT TGTCTGACAAAAGAATGAACAATTTGCTGAATAAAAGCCCCAAGTCAGGATATATACACAGAAGAAATGGAGCCTCAGAG TCTCAGCACTTGTGCNCAATGAAAGGAGAAATCTTTAAAAAAAAAAATCTGTAACAGAGGATAAAAATCAAAAGGCAGCA AGGACACCGACACTGGNGAGGTGGGAAAGGGGCTNNTCANCTACCAGCATGGGCTCNNCNGCTAGAGAGCTAACCNATNT CAAGGATTTTCAACNCTGAGGTGGGTTCCANNTCCCCTGNN
RNA-8: spliced NUCKS1, 99.7% >RNA-8-M13R_F08.ab1 NNNNNNNNNNNNNNNNNNNNNNTCGGNNCCNCTAGTNNCGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCACT ATAGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTCGAACTGCCATAGTCACTATCGTCATCATCTTCCATTAGGAAAT CTTCATCGCTGCCGGAATTCTCCTGGAATGGTGCCTCATCCTCCTCTTCTTGTTCTTCCTCACTGCCCACATCTTCCATG AGCATCTCTCTCTGTTTAGAAGCTGCTTTCGATGCCGCCTGCCGTTGTTGGCGCACATTTTTATGATCTTCTTTTTCATC TTCACTATCCTCTGCTGAGTGAGAATCATCCTTCTTGGTCTTCACATCTTTGTCTTCTGAGTCCTCACTATCTTCCTGTG AATTCTTTCCAGATCGCCTCTTATTTTTAGCTTCTCGGGGAGATGATCGAATTTTCTTAGTGGGAGGGCCCGAATCTCTT CCATAATCTTCATCTGCNTCATCAGATTCCTGAAACTGTGAGTAATCAACAACCTTCCTATTTCTGACAGGCCGCGACAT GTTCGCTGTCGAAACAGGACCGAGTCGAGAAGCCAAAGACCAGGACCCCCCCTACCCCGCGCGCTCGGCGCCCCACCCAA AANNNANNNAAANANAAAAGGGATACCCCTATNNNNNNTCGTATTNAAGCCNNNNNNNNGCAAGATATCCATCACACTGG NCGGCCGCTCGAGCATGCATCTAGAGGGNCCCAATTTCNCCCCTAT
RNA-9: no priming
RNA-10: spliced EST, 99.0% >RNA-10-M13R_F10.ab1 NNNNNNNNNNNNGNNNNNNNNNNNNCNNNNNNNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCA CTATNGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTCCTAGCTCATCCTGGCTCAAAAAGCACCCCCACTGA GCACCTTGCGACCCCCACTCCTGCCCGCCAGAGAACAAACCCCCTTTGACTGTAATTTTCCTTTACCTACCCAAATCCTA TAAAACAGCTCCACCCCTATCTCCCTTCACTGACTCTCTTTTCGGACTCGGCCCCCCTGCACCCAGGTGAAATAAACAGC CATGTTGCTCACACAAAGCCTGTTTGGTGATCTCTTCACATGGATGCACATGAAATTTGGTGCTGTGACTCAAATCGGGG GACCTCCCTTGGGAGATCAATCCCCTGTACTCCTGTTCTTTGCTCCATGACAAAGATCCACCTATGACCTCAGGTCCTCA GACCAACCANCCCAAGGAACATCTCACCGATTGTAAATCAGGTGAAGTATGAAGTTATGAGGAGAAAGGAACAGAGGACA ACCACACAGAGAAAGTGTAGCTTTATCTACTAGAGCTGAAGACATTCAAATTTATGATCCAACAATTCTACTCTGAAGTG CAGTGGTGNATATACTGCAACAATGTGTACTCATGTGCCCTGAGAACTANNNNTGAAAAAAACCTTACAGCAATATGCNT AATANNNNNNAAAAAAAAAAAAAAAAAAAANGGGATNCCCCCTATNGNNGNNTCNNNNTTAAAGCCAAATTCTGGCNGAN ANTNCNTCANN
RNA-11: PQ, CMTM3, 97.5% >RNA-11-M13R_F11.ab1 NNNNNNNNNNNNNNNNNNNANCNNNGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTATACGACTCACTAT AGGGGTANCCTTTTTTTTTTTTTTTTTTTTTTTTTGTCAGTCTGGCTTAATTCATTATGGCTTTTTTTCAGGGTAAGAAA TTCACACCCAAGAGAATAAGGCTACAAATGCTCCAAACATGGGTCCAGAACTCCGGCTCTTTTGTGAGGCCTAGAGTCAC AAAAAATGCCATCTGTTAAAAAGCTGCCTTTGCATAAAAAAATATAAAAAGTTACAAAATTCCATCAAAGACAGATACAC AGTATAAATCGTTAGGTGAACACAGAAAGCTGGTCTCATCTCCTGCAGTGGCCCATAAACCTGCCCTGANGTCTGGAAAT TCACTGTCCTGAGGGTCCAGGAACTCCTGCCTGCTGGCTGGGCTCTAGGAAGAAGGAGGGTTTGGCTGGGCCTGCCCAGC TCCCCATGACACTGTCACTGTGATGCCACTGTTGTACCCTCTGTCTTCTGGACCAGTCAGTCTATGAGCTCTTCTTTGAA ACAGTCGTAGACCCAGAATGGTCATCTCACCCCCAGAANACATGGTTTCCAAGGGTGGGAGGTGTTAGTTATGGGTGCCG AGCCCCACTTCCTGGGCGACAAGGCAAAAGGTCAATTTCCCCCTGCTTCAAGAATGAAGAGGGAACTGAAAAAAAAAAAA AAAAAAAAAAAAAAGGATACCCCTAANANTGAGTCNTATTAAANCCGAATTCTGCAANAATCCATNACNANTGGNGGCNG CTCGAANNNGCATTTAGAGGGNCCCNATNTCGCCCCTATANTGAGGTCNNAATANNAANTCACTGGCCCNTCNTTTTAAC AACNNTCNNTGNANNGGGAAAAACCCTGGCNGTTANCCCAATNTTNANTCGCCCTTGNAAGCANNNNCCCCCCTTTTNCC AGNCTGGGNGTAATAACCGAAAAGNNCCCNCANCNNNNCGCCCNTTCCCNANAGTTGCGCNACCNGANANNGNNAATGGG NCNGCNCCCNGNNNNCGGCGNNNTTATANNNNNGNNNNNNNNGN
RNA-12: PQ, HN1, 98.9% >RNA-12-M13R_F12.ab1 NNNNNNNNNNNNNNGNNNNNNNNNNTCGGANCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTC ACTNNNAGGNGATNNTTTTTTTTTTTTTTTTTTTTTTTTTTTACNNGTCCTAAACTACTAAACCTGCATTAAAAATTTCG GTTGGGGCGACCTCGGANCAAAACCCAACCTCCGANCAGTACNTGCTAAGACTTCACCAGTCAAAGCGAACTACTATACT CAATTGATCCAATAACTTGNCCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATTCTATTCTAGAGTCCATATCAAC AATAGGGTTTACGACCTCNATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATT AAAGTCCTACGTGATCTGAGTTCAGACCGGAGNAATCCNNGTCGGTTTNTATCTACTTCAAATTCCTCCCTGTACGAAAG GACAAGAGAAATAAGGNCTACTTCACATAGNGCCTTCCCCCGTAAATGANATCATCTCAACTTAGTATNNTACCCACACC C
RNA-13: no priming
RNA-14: PQ, spliced TVC (truncated?) 87.6% >RNA-14-M13R_G02.ab1 NNNNNNNNNNNNNNNNNNNNNNTCGGNNNNNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCACT ATAGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAAAGTACTGTGCTTTTATTAAAAAACAAAACAAAACAA AAAAAATACAATCAGGTACTGTCCAGACANGTTTGGGAAGGGAAAATCTCTTTTAAAGTCCTCAGTTTTCATCATCACCA TCACCATTATTATATTAATAATATTAATGTCCTTGAAATTTAAACAATATCACTTTTCTGGCTTCAGTTGTAGGAAATGT ACAAAACATCGAGGGTGGGGGTGGGGCAAGGGGTAACTTCCAATGACACAAGTTTCATCTTTGTGACCTCAACCAATAAC AATTGNACAGCTTTGTGTAAAACACAGCTGGCTCGGAGAATGACCATGTCCCCTGTATGGCCAGCTTGCCCCTCTCCCAT TCCCAGAGCCACCCAAGAGAAGTAAAATTCATGGTGTTGCTGTCACTAGGAGACTTTGCTCAATCGAACAAAAAAGTAGA AACTTGTAGACTGTTGTCTTTGGTGCAAAGGAAGTCGTTGCAGAGGGGCCTACCTCAGTGCCTCAGTGATCCGTGTGGTC TGCTTGGACGCCCATCAAATGCCCAAGGAGAGCTTCNNTGTNNNNNNTGGNTGTGTCGATGCTTTGGNAAAAATCCTTTC TTTTCTCTGGGGCCCCATTCCCTCTCTCACCNAATTATCCCTGGAATTTATGCCCNGCTTACGCCTGCCNTTCNANATTT CGGGAAGTTGCTGGGTNCTCAANTTTCANCTCACAAACTGGANTTGAGTACTCATGGCCCNTTNCAANNGGNAANCNAGT NTAANGCCCCTGACTGTNNATTATNGNNCCATATCTGCCCCATCAGGNNNCNCGATGACANTTCNNNAAAAAAAAAAAAA AAANNANAANAAAAANNGTNACCCCCTANNNNNAGTCTANTTTAAAGCCCNAATTCTCNGCNAAATANNNNTNNNNNNNG CNN
RNA-15: spliced and truncated PCNA, 88.4% >RNA-15-M13R_G03.ab1 NNNNNNNNNNNNNNNNNNNNNNGNNTCGGANCCNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTC ACTANNNGGGNTCCTTTTTTTTTTTTTTTTTTTTTTTTACTTTNAAANTTGTTAACTTTATTTAAATTGTACAATATTTC TTAAACTGCATTTAGCGTCAAGATCNNNNNNGAGAATCACCAGTATTTGTAAAGCAGAAACATTCATGTTAACAAAACTA GTTTTTACTTTGGAGCTTATTCTTTAAGCAAATTATGGAGAATGGAGAGAAAAGAAAGTAGGTTATTTACAGAAGATATC TACATATTTTATTTTAATATTTAAGAATACTCAGGTACAAACTTGGTGACAGAAAAGACCTCAGGACACGCTGGCATCTC AGGAGCAATCTTCAAAGGTTTAGTTTTCTCAATTTCTAGCAATGCCTAAGATGCTTCCTCATCTTCAATCTTGGGAGCCA AATAATACTTTAAGTGTCCCATGTCAGCAATTTTATACTCTACAACAAGGGGCACATCTGCAGACATACTGAGTGTTACT GTTGNAGACAGTGGAGTGGCTTTTGTGAAAAAGTTCAGGTACCTCANAGCAAACGTTAGGTGAACAGGCTCATTCATCTC TATGGTTACCGCCTCCTCTTCCTTATCCACATTGCTTGTTTGTGACAACTNCCATGTTCCCGTTGCCAAGCTCTCCACTT GAAGAAAACTTAAAAAAAAAAAAAAAAAAAAAAGGATANCCCTANANNGAGTCNNATTTAAAGCCNAATTCTGCANATNN
DNA samples[edit]
- 15 colonies directly for TempliPhi, roughly ~500ng/ul in 10ul PCR, 3ul sending for sequencing (M13R provided by Genewiz)
- No priming: 1
- Vector seq: 3
- Non-coding region with poly A/T: 10
DNA-1: vector seq. no A tail >DNA-1-M13R_G04.ab1 NNNNNNNNNNNNNNNNNNNNNNCTCGGATNNNCTAGTAACGGCCGCCAGTGTGCTGGAATTCTGCAGATATCCATCACAC TGNCNNCCGNNCGAGCATGCATCTAGAGGGCCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTT ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTA ATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGACGCGCCCTGTAGCGGCG CATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCT TTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATT TAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGG TTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATC TCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAANAATGAGCTGATTTAACAAAAATT TAACGCGAATTTTAACAAAATTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGTAGAAAGCCAGTCCGCAGAAACG GTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCANGTAGCTT GCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAANCGGAATTGCCAGCTGGGGCGCCC TCTGGTAAGGTTGGGAAGCCCTGCANNACNGNNGNTCTGCNCAGNNNNGNNGNNNNNNNNNNNGNNGGNNNGGGGNANNA NNNNTCTNNAANAANNANNNGNGATGNNNGNTNNNTTTCNNATNNANNGAAANAANNNNGATTGCACCGCNGGTNNCNCC NNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNACNGGGNNNNNN
DNA-2: vector; no A tail >DNA-2-M13R_G05.ab1 NNNNNNNNNNNNNNNNNNNNAGCTCGGATCCNCTAGTAACGGCCGCCAGTGTGCTGGAATTCTGCAGATATCCATCACAC TGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTT ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTA ATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGACGCGCCCTGTAGCGGCG CATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCT TTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATT TAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGG TTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATC TCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTNNAAAATGAGCTGATTTAACAAAAATT TAACGCGAATTTTAACAAAATTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGTAGAAAGCCAGTCCGCAGAAACG GTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCNNTAGCTTG CAGTGGGCTTACATGGCGATAGCTAGACTGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTC TGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCNGCCNGGATCTGATGGCGCAGGGGATCANATCT GATCAGAGACAGGATGANGNNCGTTTCCNCATGATTGAACAAGAATGGATTGCNNNNANGGTNNNNCNNNNNNTTNGGNN GNNANNGCTATNNNNNNTANNNNNN
DNA-3: Intergenic region with 20bp poly A >DNA-3-M13R_G06.ab1 NNNNNNNNNNNNNNNNCCNAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCACT ATAGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTTTCTTGAATTGTACTCCCATAATTCCCACATGTTGTGGGAGAGA GCTGGTGGGAGATAATTTGAATCATGGGGGTGGTTTCCCCCATACAGTTCTCATGGTAGTGAATAAATCTCATGAGATTG CATGGTTTTATCAGGGGTTTCTGCTTTTGTATCATCCTCGTTTTCTCTTGCCACTGCCATGTAAGAAGTGCCTTTCGGCC GGGTGCAGTAGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGTGGATCACAAGGTCAGGAGATCGATACC CTCCTGGCTAACACAGTGAGACCCCGTCTCTACTAAAAATACAAAAAATTAGCCAGGCATGGTGGTGGGCGCCTGTAGTC CCAGCTACTCAGGAGGCTGAGGCAGGAGAATGGCGTGAACCCCAGAGGCGGAGCTTGCAGTGAGCCGAGATCTCGCCACG GCACTCCAGCCTGGGTGACAGAGCGAGACTCTGTCTCAAAAAAAAAAAAAAAAAAAAAAAAGGATACCCCTNATAGTGAG TCNNNTTAAAGCCGAATTCTGCANATATCCATTCACACTGGNGGCCGCTNGAGNNTNCCNTNTAAAAGGGCCCAATTCNC CCTANANTGAGTNCGTATTAACAATTCACTGGCCGTCGTTTTACAACGNCGTGACTGGGAAAACCCTGGCGTTTCCCCAN CTTAATCCGCCTNGCANCACATCCCCCTTTCGCCAGCTGGGGNAATANCGAAANANGCCCGCACCNNTNNGCCCTTCCCA ACAGTTGCNCANCCTGAATGGNAAANGGANNGCNNCCCTTGTAGCGNNN
DNA-4: intronic region with 40bp poly T >DNA-4-M13R_G07.ab1 NNNNNNNNNNNNNNNNNNNNNNNAANCTNGNNTCCNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGAC TCACTATAGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTATCAGAAGCAAAACCACAAAATCCTCACTCTCAAGGAAT GCACAGGCTATTGCAGAAGCCAGGAAGAAATCTATATTATTACAGAGTACTTATCTCAGAGGGTTGTTATAAATATTAAA TGAGAGTTATTTTATGGAATACATCTGATCTAGTGCCTGGCACATAAAAAGTGTTATATAAGTATCAGCTATTGTAATTA CAGAGTGACAAATAAAAGGTCGGAGTGCTATGGTTATGGAATAGAATGATGAGAAAGGAAACTTCCAGGAGCTTAGGTGT ACTTTAAGTGCAGGTATCAAATATTGATGCTTCATTATGGCAAGGGGGCTAACAAGTTGCCAGTGTGTCACAAGTCAACA TGCAGGCAAAGATGGAAACATTTTCCAGATCTAAAATGTAGTTAAATATGTTGTACTTTGGAATTGTATATGAACTTTAT TCCCCTTTCAGAATTACAAATCTCTTAGAAAGCCATTTTATTTCTTTCTGAAACTGAGTTATGATCATCTACTATTTAGC ACAGAAATAAAAGGGAGGGATAAAATCTACCAACCCAGCAAAGACACATCTTGCTTNNNNNNNTCTTGAGGAAGGCCTTC AGCAGAATGGAAAGATGACTCTCTGTTGATTACTCTATGTTGAGAACCTTAAGATTCAGATGGTATAGGATTCCAGCTAG GCAGTTGTGAACTGTCCATTTGTCTTATGCAAGAAAGTCTCNTTCAGCTTTCTGAACTTTTCTGTATTTCACATACTCAT TTCTAGTTCATGCTCATGTTCAAAGCTTGCTAGAATAGCATGAAAAAGTTTCCAAAGTAAACTCAGCATACTCAAANNTT TTTCTAGGNTTATTATCTNNNNNNGGAAAGGTGAAAAGANAGCTTGCNTTTANTTTTAAACNCTTACNNNNNNNNAAAAT ANNNNNCNCTGNCANNANNNNNAAANTGACTGGNNNGNGNNCNNNCNNNANCCAGNNNTTGGNGCNANGNNGGCNNNNNN N
DNA-5: intronic region with 30 poly A >DNA-5-M13R_G08.ab1 NNNNNNNNNNNNTNGNNNNNNNNNNCGGATNNNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCA CTATANGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTACTCTTTGAGATAATACAACTGGCACAGAAAAATAAGGAGGA ACAAGAGAAAGAAAATTCCTTCAAAAGAAAGAATATATACAAAGACTCTGAGGTGGAAGAGATTGGCCTTTTCAAGAAAT TAAAAGAAAATCCACGTGACTAAAGCATAATGAGCAAAGGAAAGCCCAGTCAACAGGGGCAGATTATATAAGGTCTTGGA TAAATAGTGTCTATTTCATTCAAAGTATAATGGAAAGACACTGGAGGGTTAAAGCAAGGAAGTAAAATCATCGAATTTAC TTTTTTCTTTTTTAATTCATGAAGCTATACTGTGGAGAATGGATTATAGACGGGTTAAAGAAAAACAATGAGGCCGGGCA CAGTGGCTCATACCTATAATCCCAGCACTTTGGGAGGCCAAGGCAGGCAGATCACGAGGTCAGGAGACCAAGACCATCCT GGCCAACATGGTGAAACCCCGTCTGTACTAAAAATACAAAAATTAGCTGGGCATGGTGGCGGGTGCCTGTGATCCCAGCT ACTCAGAAGGCTGAGGCAGGAGAATCNTCTGAACCAGGGAGTCGGANATTGCAGTAAGCCGAGATCATGCCACTGCACTC CAGCTTTGGCAACAGAGTGAGACTCCCATCTCNAAAAAAAAAAAAAAAAANAAAAAAAANAAAAGGATACCCCTNTAGNG AGTCGTATTNAAGCCGAAATTCTGCANATATTCCATCACACTGGGNGG
DNA-6: interginc region with 30 poly A >DNA-6-M13R_G09.ab1 NNNNNNNNNNNNNNNNNNNNAGCTCGNNNCCNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCAC TATAGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTCAAAACTAAAATGAAATATTATCTCCCCCCAGTTAAAATG GCTTTTATCCAAAAAACAGGCAATAACGAACACTAGAGAGGATGTGAAGAAAAGGGAACCCTCGTACACTGTTGTTGGGA ATGTAAATTAAGACAACCACTATAGAGAACAGTTTGGAGGTTCCTCAAAAAACTAAAAATAGGGGTCCAGCACGGTGGCT CACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGCGGCTCACAAAGTTAGGAGATCGAAACCATCCTGGTTGACA CAGTGAAACCCCGTCTCTACCAAAAACACAAAAAATTAGCTGGGCATGGTGGCAGGTCCCTGTAGTCCCAGCGACTCAGG AGGCTGAGGCAGGAGAATGGGGTGGACCCGGGAGGCAGAGCTTGCAGTAAGCTGAGATCACGCCACTGCACTCCAGCCTA GGCGACAGAGCGAGACTTGGTCTCAAAAAAAAAAAAAAAAAAAAAAAAGGATACCCCTATAGTGAGTCGNATTAAAGCCN AATTCTGNNAANATCCNTNACACTGGCGGCCGCTCGAGCATGCATCAAAAGGNNNCNAANTCGCCCTATNNNGANTCGNA TTNCANNTCACTGGCCGTCGTTTTACAACGTCNNACTGGNAAAACCCCTGGCGTTACCNAACTTAANNNNCTTGCAGCAC ATNCCCCCTTTCCNCCNGNTGGCGTTATANCGNAGAGGCCCNGCACCGGATCNCCCNTTCCCAANANTTNGCNNCNACCC TG
DNA-7: interginc with 15 poly T >DNA-7-M13R_G10.ab1 NNNNNNNNNNNNNNNNNNNNNNNNNTCNNNNCCNNTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTC ACNANNGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTAAGTATATTTTAAATATCCTAAACAAAATATTAGC AAAGTGAATCTAACAAACATACTTAACATTTTGTTGAGAATATTCTTATAGAAAGAATAATACACCACAAACAAGTGGGA TTTATTTCAGGGATGCAAGGCTGGTTCAACATTTAAAAATCAGTTAATGTTATCCATCACACCAACAAGCTAAAAAGGAA CAGTCATATATTCATATTAATAAATGAAGAAAAAGCAGTTGAAAAAATTCAACATTCAAGATTTAAAAAACAACAAATTA GGAGAGGACTTCCTCGACTTGTTAAGGAACATCTATAAAAAACCTATAGCTAACATCATATATAATGGTGAGAAACACAA ATCTCTTACATTAAGATCAGGGAAAAAGCAAGACTGTTCTCTGTCCCCACAGCTTTTCAACATTATATAGGAAGTTTAAG CTAATGCAATGAAAAAGAAAAGAAAAGGTAGATAAATTGGAAAAGGAGAAATTAAACTGTCTTTGTTTTCAGGTGACATA TTTACCTATGGAGAATATCCAAAGAAGCTGACGAGAAAAAAAACCTGAAACCCATAAGCAATTAGGTTTCAGGATAAACG GTTAATGTACAAAAGACAATTACTTTTTTATGCACCATCAATGGAAAATGAGACTTGAATTGTAAAATACAGTATCACTC ATATTAGCACCCAAAAATGAATAGAAANTAAATCAAGCAACATATGTACNAGATCTATATGAGAAAAACTATAAAAATCT GANCAAAAATATATCTAAGAAGAANCTAAATAAANNAAGAGAGTCCATGNTTCATGGANNAGAAANGACTAAANATNGTC AACTTGTCAGTTTCCNTNCCNNACTTGATCTACAGACNTNCACAAAAATCCTNCAGTCNAAAACCNNNCANCAAANTNTT NNNNNNNNNNNNNCCNNNNNAANNTGGATNCCNNNANNNNNATNNNNNNNAANANNCNGNANNNGCTNATNCNANACTNA AANNNNNGNAAATNNNNNNNTNNNN
DNA-8: intronic region, 16 poly A >DNA-8-M13R_G11.ab1 NNNNNNNNNNNNNNNNNNNNGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCACT ATAGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTCGGCATTTCGGAGGCCTAGGTGGGTGGATCACTTGAGGTCAGGA GTTCAAGACCAGTCTGGCCAACATGGTGAAACCCCATCNCNACTAAAAATACAAAAATTAGCAGGGCGTGGTGGCAGGCC CTTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCCCTTGAACCTGGGAGGCGGAGGTTGCAGTAAGCTGAGAT TGCACCACTGAACTCCAGCCTGGCTGATAAAACAAGACTTCAACTAAAAAAAAAAAAAAAAAAANAAAAGGATACCCCAA TA
DNA-9: intronic with 25 poly T >DNA-9-M13R_G12.ab1 NNNNNNNNNNNNNNNNNNNNNNCNNGNATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCAC TATAGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTTTGAGACAGTCTNGGTCTGTTGCCCAGGCTGGAGCACANCGGC NTGATCCTGGCTCACTGCAGCCTCCACCTCTGAGGTTCAGCAATTCTCCTGCTTNNGCTTGAGTAGCTGGTACTACAGGN ACATGCCACCATGCCCAGCTAATTTTTGTATTTTTGGTAAAGACAGGGTTTCGCCATGTTGGCCAGTCTGGTCTTGAANN CCTGACTTCAAGTGATCCACCAGCCTCAGCCTCCCAAAGTGG
DNA-10: no priming
DNA-11: intergenic with 22 poly A >DNA-11-M13R_H02.ab1 NNNNNNNNNNNNNNNNNNNNNNNGNNTCGNNNNNCACTAGTAACGGCCCGCNNAGTGTGNTGGAATTCGGCTTTAATACG ACTCACTNNNGNTNNTNTCCTTTTTTTTTTTTTTTTTTTTTTTTTATCTTCTGTTATAGTCACAATAACATGTTATGCCT TTGCTTGTATGTTCATCTCTCTCTGTGTACTAGCAGTCCATCCTAATAATGATCTTATCATCTGGAATATAATTGAATAT TACAATTGAGAATAAAATAGATTATCCAGATTATTTCAGTGAATATAAGTCTAGCTGACTAAGAGGGAAAAATAATTATA GATACCGTGACATCCCCACACCAACTTCTTGTAACAATGAGATGAGTGAAACTTTAATGAAACAGTGTTATCATCAAAGA CCTGCCAACCTAAAATTATCTGTTTTGATGAATTGTCATTAAATAAATGAAGACGCCAAAATAAAGATATTTTATACAAA AACTAAATCCCTTATTCCCTCAGAGAGTTTGTAGGTTTCATTCCAAAAAGCCACAATAAAGTGAATATTGCAATAAAGCA AGTCACACAAATTTTTTGGTTTCCCAGTGCATATAAAAGTTATGCTTGGGGCTGGGCACGGTGGCTCACGCCTGTAATCC CAGCTCTTTGGGAGGCCGAGGCAGGCGGATCACAAGGTCNNNNGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGT CTCTATTGAAAATACAAAAAATTAGTCGGGCGCAGTGGCGGGCGNCTGTAGTCCCAGCTACTGGGGAGGCTGAGGCAGGA NAATGGTGNCAACCNGGGGGGCGGGAGCTTGCAGTGAGCCGAGATTGCGCCACTGCACTCCAGCCTGGTGACAGANCGAG ACTGCNTCTCNAAANAAAAANAAANNANAAAAANGNNACCCCNATAGNNGNGTCGTATAAANNCNNATTCTGCNNATNTC ATTNNCANNNNCGGNCNGCTCNAGCATGCATCTAGAGGGCCNAATTNNNCCTNTANNNAGTCGTATTANATTNACTGGCN GTNNTTTTANNANNGTNNNNGACNNNGGAAAANCCTGNNN
DNA-12: intergenic with 23 poly T >DNA-12-M13R_H03.ab1 NNNNNNNNNNNNTNGNNNNNNNNNTNGNNNNNNNANTANCGGCCGCCNGTGTGCTGGAATTCGGCTTTAATACGACTCAN NNTANNGGNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTGANACAAGAGTNTCCCTCTGTCATCCAGGCTGGAGTGCAGTG GCGTGATGTTGGCTCACTGCAACCTCTGCCTGCCAGGTTCAAGCAATTCTCCTGCCTCAGCCTTCTGAGTAGCTAGGATT GCAAGCACATACCACCATACCCGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGATGGTCT CAATCTCTTGACCTCGGGATCTGCCCGCCTTGGCCTCCCAAAGTGCTGGGATTACACGCNTGAGCCACCATGCATGGCCA AAAGCTGCTACNTCTAAAGGTGTTTCTTAGAAATAGCAAAGNANTATAGCCAGGAAAAACAATTACAACAGCCACAAACT CTAN
DNA-13: vector seq. >DNA-13-M13R_H04.ab1 NNNNNNNNNNNNNNNNNNNNNNNNCTCNNNNNNNCTAGTAACGGCCGCCAGTGTGCTGGAATTCTGCAGATATCCATCAC ACTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTT TTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGACGCGCCCTGTAGCGG CGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCG CTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGA TTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGAC GGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTA TCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCNNTTGGTTAAAAAATGAGCTGATTTAACAAAAA TTTAACGCGAATTTTAACAAAATTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGTAGAAAGCCAGTCCGCAGAAA CGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCANGTAGC TTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCNGGTTTTATGGACAGCAAGCGAAACCGGAATTGCCAGCTGGGGC GCCCTCTGNTAAGGTGGGAAGCCCTGCAAAGTAAACTGGAATNGCTTCTTGCCGCCAAGGATCTGATGGCGCANGGGATC AAGAANCTGATCAANNANANNGNATGANGNATCGTTTCGCATGAATGAANAANAATGGAATTGCNCNCAGGNTNNNCNGN N
DNA-14: intergenic region with 16 poly T/G >DNA-14-M13R_H05.ab1 NNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNTCCACTAGTAACGGCCGCNAGTGTGCTGGAATTCGGCTTTAATACGA CTCACTATNGGGGNNTCCNTTTTTTTTNTTTTTTTTTTTTTTGAGACAGAGTCTCGCTCTGTCCCCAAGTTGGAGTGNNN TGGCCCGN
DNA-15: intergenic region >DNA-15-M13R_H06.ab1 NNNNNNNNNNNNNNNNACNAGNNNGGANCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCNCN NNANNGNNNNGCNTTTTTTTTTTTTTTTTTTTTTTTCTCNGCNANNACCNNNAAATATCATGGTCTCTGGCAAGATGTCC AAAATCTGATTCAGGTCTTCCGGTCAGTCGTAGCCAGCTATCA