Rui:LabNotes/SingleCell/2011-6-4
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Kapa PCR for ER EL[edit]
- EL sample is on 5.26.11
- ER sample is on 6.2.11
- 2ul samples in 40ul total. EL - N2.ID12; ER - N2.ID13
QPCR curve[edit]
TBE PAGE[edit]
Size selection for sequencing[edit]
- run TBE-PAGE, cut bands around 300bp-700bp
- finish gel purification procedure in one day (exact 1hr 37C incubation of gel in TE buffer)
- Gel check for selected library was in lab note 6.6.11 [1]
- Mixed with haplotyping samples for sequencing, sample ID: RL-Hap_RNAseq-1_13-Jun6