Rui:Microscope

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Microscope[edit]

  • Turn on:
  1. Flurenscence
  2. Camera (yellow to green)
  3. Microscope
  4. Stage (on top of micro-manipulator)
  • Meta Morph

Observation

  1. Journal --> taskbars --> Load taskbars --> Main.JTB
  2. play with camera parameters (exposure time: BF-1ms; CY5-500ms; GFP-200ms)
  3. Start live, acquire for snap
  4. focus by eye, switch to camera

Timelapse

  1. Set up a Journal
  2. Acqure --> Acquire Timelapse
Time interval: 15min
Durationg: 60hrs
Image storage: Disk
Save file: ... tiff?
Journal to run for each time interval: 4X_BF-1ms_acquisition
Check: update image window, warn if interval time exceeded
Illum: BF