Rui:SingelCell RNAseq

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SCAP project[edit]

Background[edit]

  • Goal: the smallest functional unit; the exact quantity; full-length RNAs; sense vs anti-sense; all classes; at single-base resolution
  • Review papers:
  • Key papers:
  1. Single cell and cell lysis
  2. 1st cDNA synthesis and free primer removal
  3. 2nd cDNA synthesis: A-tailing vs random
  4. Amplification: PCR or IVT
  5. QC and clone

Protocols[edit]

QuickNotes[edit]


2014[edit]

  • Reference RNA data analysis:
  • The first 1000 single-nucleus data workflow: 7.3; 7.4; 7.14; 8.7
  • The bulk sample analysis: 7.9.14; 10.10.14
  • The pipeline:

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2013[edit]

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2012[edit]

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2011[edit]

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