Rui:TotoRNAseq protocol
Jump to navigation
Jump to search
totoRNA library structure[edit]
Standard primers[edit]
totoRNAseq primers[edit]
File:Libraries totoRNA-5.jpg File:Libraries totoRNA-3.jpg
totoRNA primer sequence[edit]
- The 5' adaptors list
Tube ID | Primer full name | Primer full sequence | PE_F-N10 | Barcode | TSO |
TSO.r01 | BR_TSO_N10_BC6 | ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNdUNNNNdUNNNTTTAGGrGrGrG | ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNN/ideoxyU/NNNN/ideoxyU/NNN | TTTAGG | rGrGrG |
TSO.r02 | B_TSO_N10 | ACACTCTTTCCCTACACGACGNNNUNNNNUNNNrGrGrG | ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNN/ideoxyU/NNN | \ | rGrGrG |
TSO.r03 | B.N10.TSO.NH2 | ACACTCTTTCCCTACACGACGUNNNUNNNNUNNNrGrGrG/3AmMO/ | ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNN/ideoxyU/NNN | \ | rGrGrG |
TSO.id01 | B.TSO.N10.BC8.Idx1 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTCTGAGACrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | TCTGAGAC | rGrGrG |
TSO.id02 | B.TSO.N10.BC8.Idx2 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTGCCCAAGrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | TGCCCAAG | rGrGrG |
TSO.id03 | B.TSO.N10.BC8.Idx3 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGACTCGCTrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | GACTCGCT | rGrGrG |
TSO.id04 | B.TSO.N10.BC8.Idx4 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCCACTCCArGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | CCACTCCA | rGrGrG |
TSO.id05 | B.TSO.N10.BC8.Idx5 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGAACAGGGrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | GAACAGGG | rGrGrG |
TSO.id06 | B.TSO.N10.BC8.Idx6 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCTGCTGATrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | CTGCTGAT | rGrGrG |
TSO.id07 | B.TSO.N10.BC8.Idx7 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCTAGTATCrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | CTAGTATC | rGrGrG |
TSO.id08 | B.TSO.N10.BC8.Idx8 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGGCTGAAArGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | GGCTGAAA | rGrGrG |
TSO.id09 | B.TSO.N10.BC8.Idx9 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGAGTGCTTrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | GAGTGCTT | rGrGrG |
TSO.id10 | B.TSO.N10.BC8.Idx10 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNATACGCCArGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | ATACGCCA | rGrGrG |
TSO.id11 | B.TSO.N10.BC8.Idx11 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNATGCGTTArGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | ATGCGTTA | rGrGrG |
TSO.id12 | B.TSO.N10.BC8.Idx12 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTCACCCTTrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | TCACCCTT | rGrGrG |
TSO.id13 | B.TSO.N10.BC8.Idx13 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCCAACCGCrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | CCAACCGC | rGrGrG |
TSO.id14 | B.TSO.N10.BC8.Idx14 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGGATTACCrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | GGATTACC | rGrGrG |
TSO.id15 | B.TSO.N10.BC8.Idx15 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCAGAGACArGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | CAGAGACA | rGrGrG |
TSO.id16 | B.TSO.N10.BC8.Idx16 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCAATAGTGrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | CAATAGTG | rGrGrG |
TSO.id17 | B.TSO.N10.BC8.Idx17 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGGTGGCCArGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | GGTGGCCA | rGrGrG |
TSO.id18 | B.TSO.N10.BC8.Idx18 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGGAGGGAGrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | GGAGGGAG | rGrGrG |
TSO.id19 | B.TSO.N10.BC8.Idx19 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGTTGTCCTrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | GTTGTCCT | rGrGrG |
TSO.id20 | B.TSO.N10.BC8.Idx20 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTTCGACCTrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | TTCGACCT | rGrGrG |
TSO.id21 | B.TSO.N10.BC8.Idx21 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGCTTATTGrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | GCTTATTG | rGrGrG |
TSO.id22 | B.TSO.N10.BC8.Idx22 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCAACCTTTrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | CAACCTTT | rGrGrG |
TSO.id23 | B.TSO.N10.BC8.Idx23 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCTTAGGTArGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | CTTAGGTA | rGrGrG |
TSO.id24 | B.TSO.N10.BC8.Idx24 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGCTCACCArGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | GCTCACCA | rGrGrG |
TSO.id25 | B.TSO.N10.BC8.Idx25 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNAGAAATGCrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | AGAAATGC | rGrGrG |
TSO.id26 | B.TSO.N10.BC8.Idx26 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCTTACTGArGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | CTTACTGA | rGrGrG |
TSO.id27 | B.TSO.N10.BC8.Idx27 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCTCACGGGrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | CTCACGGG | rGrGrG |
TSO.id28 | B.TSO.N10.BC8.Idx28 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCACTGTCArGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | CACTGTCA | rGrGrG |
TSO.id29 | B.TSO.N10.BC8.Idx29 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTTCTTGGGrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | TTCTTGGG | rGrGrG |
TSO.id30 | B.TSO.N10.BC8.Idx30 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNACCGGCTTrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | ACCGGCTT | rGrGrG |
TSO.id31 | B.TSO.N10.BC8.Idx31 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTAACCACArGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | TAACCACA | rGrGrG |
TSO.id32 | B.TSO.N10.BC8.Idx32 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTACGCGCGrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | TACGCGCG | rGrGrG |
TSO.id33 | B.TSO.N10.BC8.Idx33 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCCTCGATGrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | CCTCGATG | rGrGrG |
TSO.id34 | B.TSO.N10.BC8.Idx34 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNAGAGCTCArGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | AGAGCTCA | rGrGrG |
TSO.id35 | B.TSO.N10.BC8.Idx35 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTATGGTCCrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | TATGGTCC | rGrGrG |
TSO.id36 | B.TSO.N10.BC8.Idx36 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTGTCCGGCrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | TGTCCGGC | rGrGrG |
TSO.id37 | B.TSO.N10.BC8.Idx37 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNAAGGAATCrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | AAGGAATC | rGrGrG |
TSO.id38 | B.TSO.N10.BC8.Idx38 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCATCGTAGrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | CATCGTAG | rGrGrG |
TSO.id39 | B.TSO.N10.BC8.Idx39 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGTGTTGAGrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | GTGTTGAG | rGrGrG |
TSO.id40 | B.TSO.N10.BC8.Idx40 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNATGTATCCrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | ATGTATCC | rGrGrG |
TSO.id41 | B.TSO.N10.BC8.Idx41 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTGACAGGTrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | TGACAGGT | rGrGrG |
TSO.id42 | B.TSO.N10.BC8.Idx42 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTACGCCAArGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | TACGCCAA | rGrGrG |
TSO.id43 | B.TSO.N10.BC8.Idx43 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGAAGGCAArGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | GAAGGCAA | rGrGrG |
TSO.id44 | B.TSO.N10.BC8.Idx44 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCACCCGAGrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | CACCCGAG | rGrGrG |
TSO.id45 | B.TSO.N10.BC8.Idx45 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGGTGTGTCrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | GGTGTGTC | rGrGrG |
TSO.id46 | B.TSO.N10.BC8.Idx46 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTCTTGTGCrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | TCTTGTGC | rGrGrG |
TSO.id47 | B.TSO.N10.BC8.Idx47 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNAGTACCTGrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | AGTACCTG | rGrGrG |
TSO.id48 | B.TSO.N10.BC8.Idx48 | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNAGCGTCCTrGrGrG | ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN | AGCGTCCT | rGrGrG |
PB_PCR.F | PB_PCR.F | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG |
- The 3' adaptor list
Tube ID | Primer full name | Primer full sequence | PE_R | Barcode | T20VN |
T20.r01 | T20VN_PE_R | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTVN | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | \ | TTTTTTTTTTTTTTTTTTTTVN |
T20.r02 | N9_PE_R | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTNNNNNNNNN | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | \ | NNNNNNNNN |
T20.r03 | T20VN_BC6_PE_R | GCATTCCTGCTGAACCGCTCTTTTTAGGTTTTTTTTTTTTTTTTTTTTTVN | /5Biosg/GCATTCCTGCTGAACCGCTCTT | TTTAGG | TTTTTTTTTTTTTTTTTTTTVN |
T20.id01 | T20VN.BC8.PE_R.Indx1 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCTGAGACTTTTTTTTTTTTTTTTTTTTVN | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | TCTGAGAC | TTTTTTTTTTTTTTTTTTTTVN |
T20.id02 | T20VN.BC8.PE_R.Indx2 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCCCAAGTTTTTTTTTTTTTTTTTTTTVN | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | TGCCCAAG | TTTTTTTTTTTTTTTTTTTTVN |
T20.id03 | T20VN.BC8.PE_R.Indx3 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGACTCGCTTTTTTTTTTTTTTTTTTTTTVN | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | GACTCGCT | TTTTTTTTTTTTTTTTTTTTVN |
T20.id04 | T20VN.BC8.PE_R.Indx4 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCACTCCATTTTTTTTTTTTTTTTTTTTVN | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | CCACTCCA | TTTTTTTTTTTTTTTTTTTTVN |
T20.id05 | T20VN.BC8.PE_R.Indx5 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAACAGGGTTTTTTTTTTTTTTTTTTTTVN | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | GAACAGGG | TTTTTTTTTTTTTTTTTTTTVN |
T20.id06 | T20VN.BC8.PE_R.Indx6 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTGCTGATTTTTTTTTTTTTTTTTTTTTVN | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | CTGCTGAT | TTTTTTTTTTTTTTTTTTTTVN |
T20.id07 | T20VN.BC8.PE_R.Indx7 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTAGTATCTTTTTTTTTTTTTTTTTTTTVN | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | CTAGTATC | TTTTTTTTTTTTTTTTTTTTVN |
T20.id08 | T20VN.BC8.PE_R.Indx8 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGCTGAAATTTTTTTTTTTTTTTTTTTTVN | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | GGCTGAAA | TTTTTTTTTTTTTTTTTTTTVN |
T20.id09 | T20VN.BC8.PE_R.Indx9 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGTGCTTTTTTTTTTTTTTTTTTTTTTVN | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | GAGTGCTT | TTTTTTTTTTTTTTTTTTTTVN |
T20.id10 | T20VN.BC8.PE_R.Indx10 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATACGCCATTTTTTTTTTTTTTTTTTTTVN | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | ATACGCCA | TTTTTTTTTTTTTTTTTTTTVN |
T20.id11 | T20VN.BC8.PE_R.Indx11 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGCGTTATTTTTTTTTTTTTTTTTTTTVN | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | ATGCGTTA | TTTTTTTTTTTTTTTTTTTTVN |
T20.id12 | T20VN.BC8.PE_R.Indx12 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCACCCTTTTTTTTTTTTTTTTTTTTTTVN | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | TCACCCTT | TTTTTTTTTTTTTTTTTTTTVN |
T20.id13 | T20V.BC8.PE_R.Indx13 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCAACCGCTTTTTTTTTTTTTTTTTTTTV | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | CCAACCGC | TTTTTTTTTTTTTTTTTTTTV |
T20.id14 | T20V.BC8.PE_R.Indx14 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGATTACCTTTTTTTTTTTTTTTTTTTTV | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | GGATTACC | TTTTTTTTTTTTTTTTTTTTV |
T20.id15 | T20V.BC8.PE_R.Indx15 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAGAGACATTTTTTTTTTTTTTTTTTTTV | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | CAGAGACA | TTTTTTTTTTTTTTTTTTTTV |
T20.id16 | T20V.BC8.PE_R.Indx16 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAATAGTGTTTTTTTTTTTTTTTTTTTTV | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | CAATAGTG | TTTTTTTTTTTTTTTTTTTTV |
T20.id17 | T20V.BC8.PE_R.Indx17 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGTGGCCATTTTTTTTTTTTTTTTTTTTV | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | GGTGGCCA | TTTTTTTTTTTTTTTTTTTTV |
T20.id18 | T20V.BC8.PE_R.Indx18 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGAGGGAGTTTTTTTTTTTTTTTTTTTTV | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | GGAGGGAG | TTTTTTTTTTTTTTTTTTTTV |
T20.id19 | T20V.BC8.PE_R.Indx19 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTTGTCCTTTTTTTTTTTTTTTTTTTTTV | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | GTTGTCCT | TTTTTTTTTTTTTTTTTTTTV |
T20.id20 | T20V.BC8.PE_R.Indx20 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTCGACCTTTTTTTTTTTTTTTTTTTTTV | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | TTCGACCT | TTTTTTTTTTTTTTTTTTTTV |
T20.id21 | T20V.BC8.PE_R.Indx21 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTTATTGTTTTTTTTTTTTTTTTTTTTV | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | GCTTATTG | TTTTTTTTTTTTTTTTTTTTV |
T20.id22 | T20V.BC8.PE_R.Indx22 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAACCTTTTTTTTTTTTTTTTTTTTTTTV | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | CAACCTTT | TTTTTTTTTTTTTTTTTTTTV |
T20.id23 | T20V.BC8.PE_R.Indx23 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTTAGGTATTTTTTTTTTTTTTTTTTTTV | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | CTTAGGTA | TTTTTTTTTTTTTTTTTTTTV |
T20.id24 | T20V.BC8.PE_R.Indx24 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTCACCATTTTTTTTTTTTTTTTTTTTV | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | GCTCACCA | TTTTTTTTTTTTTTTTTTTTV |
N2.id73 | PCR_R.N2Ind73 | CAAGCAGAAGACGGCATACGAGATGAGTCCCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | GAGTCC | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id74 | PCR_R.N2Ind74 | CAAGCAGAAGACGGCATACGAGATTCGCCACTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | TCGCCA | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id75 | PCR_R.N2Ind75 | CAAGCAGAAGACGGCATACGAGATAAGTCGCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | AAGTCG | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id76 | PCR_R.N2Ind76 | CAAGCAGAAGACGGCATACGAGATAATAGGCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | AATAGG | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id77 | PCR_R.N2Ind77 | CAAGCAGAAGACGGCATACGAGATACCCGTCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | ACCCGT | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id78 | PCR_R.N2Ind78 | CAAGCAGAAGACGGCATACGAGATAACACGCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | AACACG | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id79 | PCR_R.N2Ind79 | CAAGCAGAAGACGGCATACGAGATGCTTGGCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | GCTTGG | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id80 | PCR_R.N2Ind80 | CAAGCAGAAGACGGCATACGAGATTTACCACTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | TTACCA | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id81 | PCR_R.N2Ind81 | CAAGCAGAAGACGGCATACGAGATCCAGGTCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | CCAGGT | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id82 | PCR_R.N2Ind82 | CAAGCAGAAGACGGCATACGAGATCGTTTGCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | CGTTTG | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id83 | PCR_R.N2Ind83 | CAAGCAGAAGACGGCATACGAGATGACCACCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | GACCAC | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id84 | PCR_R.N2Ind84 | CAAGCAGAAGACGGCATACGAGATACAAGACTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | ACAAGA | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id85 | PCR_R.N2Ind85 | CAAGCAGAAGACGGCATACGAGATACCGCACTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | ACCGCA | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id86 | PCR_R.N2Ind86 | CAAGCAGAAGACGGCATACGAGATTGGGTACTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | TGGGTA | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id87 | PCR_R.N2Ind87 | CAAGCAGAAGACGGCATACGAGATATTCCGCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | ATTCCG | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id88 | PCR_R.N2Ind88 | CAAGCAGAAGACGGCATACGAGATGAATGTCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | GAATGT | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id89 | PCR_R.N2Ind89 | CAAGCAGAAGACGGCATACGAGATGCTGATCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | GCTGAT | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id90 | PCR_R.N2Ind90 | CAAGCAGAAGACGGCATACGAGATAGTGCTCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | AGTGCT | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id91 | PCR_R.N2Ind91 | CAAGCAGAAGACGGCATACGAGATCAGGGACTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | CAGGGA | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id92 | PCR_R.N2Ind92 | CAAGCAGAAGACGGCATACGAGATCATGCGCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | CATGCG | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id93 | PCR_R.N2Ind93 | CAAGCAGAAGACGGCATACGAGATTGCCTACTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | TGCCTA | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id94 | PCR_R.N2Ind94 | CAAGCAGAAGACGGCATACGAGATCTATACCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | CTATAC | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id95 | PCR_R.N2Ind95 | CAAGCAGAAGACGGCATACGAGATCCGAGTCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | CCGAGT | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id96 | PCR_R.N2Ind96 | CAAGCAGAAGACGGCATACGAGATACCTGCCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | ACCTGC | CTCGGCATTCCTGCTGAACCGCTCTT |
Sequencing primers[edit]
totoRNAseq Read 1: [1]
CACCGAGATCTACACTCTTTCCCTACACGACGTT 34nt Tm=64(IDT) Tm=71.72(Kun's) correspond to TSO.idXX primers used for 5'end
totoRNAseq Read 1 v2:
CCACCGAGATCTACACTCTTTCCCTACACGACG 33nt Tm=64.5(IDT) Tm=72.04(Kun's) correspond to TSO.r02/TSO.r04 primers used for 5'end