Rui:TotoRNAseq protocol

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totoRNA library structure[edit]

Standard primers[edit]

File:Libraries ILMN.jpg

totoRNAseq primers[edit]

File:Libraries totoRNA-5.jpg File:Libraries totoRNA-3.jpg


totoRNA primer sequence[edit]

  • The 5' adaptors list
Tube ID Primer full name Primer full sequence PE_F-N10 Barcode TSO
TSO.r01 BR_TSO_N10_BC6 ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNdUNNNNdUNNNTTTAGGrGrGrG ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNN/ideoxyU/NNNN/ideoxyU/NNN TTTAGG rGrGrG
TSO.r02 B_TSO_N10 ACACTCTTTCCCTACACGACGNNNUNNNNUNNNrGrGrG ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNN/ideoxyU/NNN \ rGrGrG
TSO.r03 B.N10.TSO.NH2 ACACTCTTTCCCTACACGACGUNNNUNNNNUNNNrGrGrG/3AmMO/ ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNN/ideoxyU/NNN \ rGrGrG
TSO.id01 B.TSO.N10.BC8.Idx1 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTCTGAGACrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TCTGAGAC rGrGrG
TSO.id02 B.TSO.N10.BC8.Idx2 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTGCCCAAGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TGCCCAAG rGrGrG
TSO.id03 B.TSO.N10.BC8.Idx3 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGACTCGCTrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GACTCGCT rGrGrG
TSO.id04 B.TSO.N10.BC8.Idx4 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCCACTCCArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CCACTCCA rGrGrG
TSO.id05 B.TSO.N10.BC8.Idx5 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGAACAGGGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GAACAGGG rGrGrG
TSO.id06 B.TSO.N10.BC8.Idx6 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCTGCTGATrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CTGCTGAT rGrGrG
TSO.id07 B.TSO.N10.BC8.Idx7 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCTAGTATCrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CTAGTATC rGrGrG
TSO.id08 B.TSO.N10.BC8.Idx8 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGGCTGAAArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GGCTGAAA rGrGrG
TSO.id09 B.TSO.N10.BC8.Idx9 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGAGTGCTTrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GAGTGCTT rGrGrG
TSO.id10 B.TSO.N10.BC8.Idx10 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNATACGCCArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN ATACGCCA rGrGrG
TSO.id11 B.TSO.N10.BC8.Idx11 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNATGCGTTArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN ATGCGTTA rGrGrG
TSO.id12 B.TSO.N10.BC8.Idx12 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTCACCCTTrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TCACCCTT rGrGrG
TSO.id13 B.TSO.N10.BC8.Idx13 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCCAACCGCrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CCAACCGC rGrGrG
TSO.id14 B.TSO.N10.BC8.Idx14 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGGATTACCrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GGATTACC rGrGrG
TSO.id15 B.TSO.N10.BC8.Idx15 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCAGAGACArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CAGAGACA rGrGrG
TSO.id16 B.TSO.N10.BC8.Idx16 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCAATAGTGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CAATAGTG rGrGrG
TSO.id17 B.TSO.N10.BC8.Idx17 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGGTGGCCArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GGTGGCCA rGrGrG
TSO.id18 B.TSO.N10.BC8.Idx18 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGGAGGGAGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GGAGGGAG rGrGrG
TSO.id19 B.TSO.N10.BC8.Idx19 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGTTGTCCTrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GTTGTCCT rGrGrG
TSO.id20 B.TSO.N10.BC8.Idx20 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTTCGACCTrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TTCGACCT rGrGrG
TSO.id21 B.TSO.N10.BC8.Idx21 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGCTTATTGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GCTTATTG rGrGrG
TSO.id22 B.TSO.N10.BC8.Idx22 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCAACCTTTrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CAACCTTT rGrGrG
TSO.id23 B.TSO.N10.BC8.Idx23 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCTTAGGTArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CTTAGGTA rGrGrG
TSO.id24 B.TSO.N10.BC8.Idx24 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGCTCACCArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GCTCACCA rGrGrG
TSO.id25 B.TSO.N10.BC8.Idx25 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNAGAAATGCrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN AGAAATGC rGrGrG
TSO.id26 B.TSO.N10.BC8.Idx26 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCTTACTGArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CTTACTGA rGrGrG
TSO.id27 B.TSO.N10.BC8.Idx27 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCTCACGGGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CTCACGGG rGrGrG
TSO.id28 B.TSO.N10.BC8.Idx28 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCACTGTCArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CACTGTCA rGrGrG
TSO.id29 B.TSO.N10.BC8.Idx29 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTTCTTGGGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TTCTTGGG rGrGrG
TSO.id30 B.TSO.N10.BC8.Idx30 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNACCGGCTTrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN ACCGGCTT rGrGrG
TSO.id31 B.TSO.N10.BC8.Idx31 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTAACCACArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TAACCACA rGrGrG
TSO.id32 B.TSO.N10.BC8.Idx32 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTACGCGCGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TACGCGCG rGrGrG
TSO.id33 B.TSO.N10.BC8.Idx33 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCCTCGATGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CCTCGATG rGrGrG
TSO.id34 B.TSO.N10.BC8.Idx34 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNAGAGCTCArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN AGAGCTCA rGrGrG
TSO.id35 B.TSO.N10.BC8.Idx35 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTATGGTCCrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TATGGTCC rGrGrG
TSO.id36 B.TSO.N10.BC8.Idx36 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTGTCCGGCrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TGTCCGGC rGrGrG
TSO.id37 B.TSO.N10.BC8.Idx37 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNAAGGAATCrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN AAGGAATC rGrGrG
TSO.id38 B.TSO.N10.BC8.Idx38 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCATCGTAGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CATCGTAG rGrGrG
TSO.id39 B.TSO.N10.BC8.Idx39 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGTGTTGAGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GTGTTGAG rGrGrG
TSO.id40 B.TSO.N10.BC8.Idx40 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNATGTATCCrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN ATGTATCC rGrGrG
TSO.id41 B.TSO.N10.BC8.Idx41 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTGACAGGTrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TGACAGGT rGrGrG
TSO.id42 B.TSO.N10.BC8.Idx42 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTACGCCAArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TACGCCAA rGrGrG
TSO.id43 B.TSO.N10.BC8.Idx43 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGAAGGCAArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GAAGGCAA rGrGrG
TSO.id44 B.TSO.N10.BC8.Idx44 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCACCCGAGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CACCCGAG rGrGrG
TSO.id45 B.TSO.N10.BC8.Idx45 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGGTGTGTCrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GGTGTGTC rGrGrG
TSO.id46 B.TSO.N10.BC8.Idx46 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTCTTGTGCrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TCTTGTGC rGrGrG
TSO.id47 B.TSO.N10.BC8.Idx47 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNAGTACCTGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN AGTACCTG rGrGrG
TSO.id48 B.TSO.N10.BC8.Idx48 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNAGCGTCCTrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN AGCGTCCT rGrGrG
PB_PCR.F PB_PCR.F AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG
  • The 3' adaptor list
Tube ID Primer full name Primer full sequence PE_R Barcode T20VN
T20.r01 T20VN_PE_R /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT \ TTTTTTTTTTTTTTTTTTTTVN
T20.r02 N9_PE_R /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTNNNNNNNNN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT \ NNNNNNNNN
T20.r03 T20VN_BC6_PE_R GCATTCCTGCTGAACCGCTCTTTTTAGGTTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTT TTTAGG TTTTTTTTTTTTTTTTTTTTVN
T20.id01 T20VN.BC8.PE_R.Indx1 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCTGAGACTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT TCTGAGAC TTTTTTTTTTTTTTTTTTTTVN
T20.id02 T20VN.BC8.PE_R.Indx2 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCCCAAGTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT TGCCCAAG TTTTTTTTTTTTTTTTTTTTVN
T20.id03 T20VN.BC8.PE_R.Indx3 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGACTCGCTTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT GACTCGCT TTTTTTTTTTTTTTTTTTTTVN
T20.id04 T20VN.BC8.PE_R.Indx4 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCACTCCATTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT CCACTCCA TTTTTTTTTTTTTTTTTTTTVN
T20.id05 T20VN.BC8.PE_R.Indx5 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAACAGGGTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT GAACAGGG TTTTTTTTTTTTTTTTTTTTVN
T20.id06 T20VN.BC8.PE_R.Indx6 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTGCTGATTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT CTGCTGAT TTTTTTTTTTTTTTTTTTTTVN
T20.id07 T20VN.BC8.PE_R.Indx7 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTAGTATCTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT CTAGTATC TTTTTTTTTTTTTTTTTTTTVN
T20.id08 T20VN.BC8.PE_R.Indx8 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGCTGAAATTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT GGCTGAAA TTTTTTTTTTTTTTTTTTTTVN
T20.id09 T20VN.BC8.PE_R.Indx9 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGTGCTTTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT GAGTGCTT TTTTTTTTTTTTTTTTTTTTVN
T20.id10 T20VN.BC8.PE_R.Indx10 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATACGCCATTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT ATACGCCA TTTTTTTTTTTTTTTTTTTTVN
T20.id11 T20VN.BC8.PE_R.Indx11 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGCGTTATTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT ATGCGTTA TTTTTTTTTTTTTTTTTTTTVN
T20.id12 T20VN.BC8.PE_R.Indx12 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCACCCTTTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT TCACCCTT TTTTTTTTTTTTTTTTTTTTVN
T20.id13 T20V.BC8.PE_R.Indx13 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCAACCGCTTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT CCAACCGC TTTTTTTTTTTTTTTTTTTTV
T20.id14 T20V.BC8.PE_R.Indx14 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGATTACCTTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT GGATTACC TTTTTTTTTTTTTTTTTTTTV
T20.id15 T20V.BC8.PE_R.Indx15 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAGAGACATTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT CAGAGACA TTTTTTTTTTTTTTTTTTTTV
T20.id16 T20V.BC8.PE_R.Indx16 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAATAGTGTTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT CAATAGTG TTTTTTTTTTTTTTTTTTTTV
T20.id17 T20V.BC8.PE_R.Indx17 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGTGGCCATTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT GGTGGCCA TTTTTTTTTTTTTTTTTTTTV
T20.id18 T20V.BC8.PE_R.Indx18 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGAGGGAGTTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT GGAGGGAG TTTTTTTTTTTTTTTTTTTTV
T20.id19 T20V.BC8.PE_R.Indx19 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTTGTCCTTTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT GTTGTCCT TTTTTTTTTTTTTTTTTTTTV
T20.id20 T20V.BC8.PE_R.Indx20 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTCGACCTTTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT TTCGACCT TTTTTTTTTTTTTTTTTTTTV
T20.id21 T20V.BC8.PE_R.Indx21 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTTATTGTTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT GCTTATTG TTTTTTTTTTTTTTTTTTTTV
T20.id22 T20V.BC8.PE_R.Indx22 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAACCTTTTTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT CAACCTTT TTTTTTTTTTTTTTTTTTTTV
T20.id23 T20V.BC8.PE_R.Indx23 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTTAGGTATTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT CTTAGGTA TTTTTTTTTTTTTTTTTTTTV
T20.id24 T20V.BC8.PE_R.Indx24 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTCACCATTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT GCTCACCA TTTTTTTTTTTTTTTTTTTTV
N2.id73 PCR_R.N2Ind73 CAAGCAGAAGACGGCATACGAGATGAGTCCCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GAGTCC CTCGGCATTCCTGCTGAACCGCTCTT
N2.id74 PCR_R.N2Ind74 CAAGCAGAAGACGGCATACGAGATTCGCCACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT TCGCCA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id75 PCR_R.N2Ind75 CAAGCAGAAGACGGCATACGAGATAAGTCGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT AAGTCG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id76 PCR_R.N2Ind76 CAAGCAGAAGACGGCATACGAGATAATAGGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT AATAGG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id77 PCR_R.N2Ind77 CAAGCAGAAGACGGCATACGAGATACCCGTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT ACCCGT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id78 PCR_R.N2Ind78 CAAGCAGAAGACGGCATACGAGATAACACGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT AACACG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id79 PCR_R.N2Ind79 CAAGCAGAAGACGGCATACGAGATGCTTGGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GCTTGG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id80 PCR_R.N2Ind80 CAAGCAGAAGACGGCATACGAGATTTACCACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT TTACCA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id81 PCR_R.N2Ind81 CAAGCAGAAGACGGCATACGAGATCCAGGTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CCAGGT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id82 PCR_R.N2Ind82 CAAGCAGAAGACGGCATACGAGATCGTTTGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CGTTTG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id83 PCR_R.N2Ind83 CAAGCAGAAGACGGCATACGAGATGACCACCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GACCAC CTCGGCATTCCTGCTGAACCGCTCTT
N2.id84 PCR_R.N2Ind84 CAAGCAGAAGACGGCATACGAGATACAAGACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT ACAAGA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id85 PCR_R.N2Ind85 CAAGCAGAAGACGGCATACGAGATACCGCACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT ACCGCA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id86 PCR_R.N2Ind86 CAAGCAGAAGACGGCATACGAGATTGGGTACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT TGGGTA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id87 PCR_R.N2Ind87 CAAGCAGAAGACGGCATACGAGATATTCCGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT ATTCCG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id88 PCR_R.N2Ind88 CAAGCAGAAGACGGCATACGAGATGAATGTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GAATGT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id89 PCR_R.N2Ind89 CAAGCAGAAGACGGCATACGAGATGCTGATCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GCTGAT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id90 PCR_R.N2Ind90 CAAGCAGAAGACGGCATACGAGATAGTGCTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT AGTGCT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id91 PCR_R.N2Ind91 CAAGCAGAAGACGGCATACGAGATCAGGGACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CAGGGA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id92 PCR_R.N2Ind92 CAAGCAGAAGACGGCATACGAGATCATGCGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CATGCG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id93 PCR_R.N2Ind93 CAAGCAGAAGACGGCATACGAGATTGCCTACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT TGCCTA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id94 PCR_R.N2Ind94 CAAGCAGAAGACGGCATACGAGATCTATACCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CTATAC CTCGGCATTCCTGCTGAACCGCTCTT
N2.id95 PCR_R.N2Ind95 CAAGCAGAAGACGGCATACGAGATCCGAGTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CCGAGT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id96 PCR_R.N2Ind96 CAAGCAGAAGACGGCATACGAGATACCTGCCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT ACCTGC CTCGGCATTCCTGCTGAACCGCTCTT

Sequencing primers[edit]

totoRNAseq Read 1: [1]

CACCGAGATCTACACTCTTTCCCTACACGACGTT
34nt 	Tm=64(IDT)	Tm=71.72(Kun's)
correspond to TSO.idXX primers used for 5'end

totoRNAseq Read 1 v2:

CCACCGAGATCTACACTCTTTCCCTACACGACG
33nt 	Tm=64.5(IDT)	Tm=72.04(Kun's)
correspond to TSO.r02/TSO.r04 primers used for 5'end