Sam:LabNotes/Microbiome-new/2010-6-9 exp1

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VA-hospital sorting on Alex-FISH E.coli (EtOH-fixed) - 30min-hybridization samples[edit]

Background[edit]

  • The new FISH-labeled E.coli was performed with only 30min hybridization (Made on 06-07-'10)
  • For this sorting, I pre-run a 1000cell sorting to cover full plate before the real sample sorting

Procedure[edit]

  • Add 900uL 1XPBS into the sample (100uL) in the amber tube.
  • Material need for sorting:
    • 8-well PCR tube stirps (on 96-well rack). Filled up 3uL 1X PBS in PCR tubes. UV-treatment for 15min.
    • UV clean sealing film (Bio-rad)
    • UV clean zip bag for transporting sample + rack
Real sorting arrangement 
             1   2   3   4   5   6   7   8   9  10  11  12 - Strip# (Strips are in vertical arrangement)
         -------------------------------------------------
         H   0   0   0   0   0   0   0   0   0   0   0   0
         G   1   1   1   1   1   1   1   1   1   1   1   1
         F   1   1   1   1   1   1   1   1   1   1   1   1
         E   1   1   1   1   1   1   1   1   1   1   1   1
         D   1   1   1   1   1   1   1   1   1   1   1   1
         C   1   1   1   1   1   1   1   1   1   1   1   1
         B  10  10  10  10  10  10  10  10  10  10  10  10
         A 100 100 100 100 100 100 100 100 100 100 100 100
         --------------------------------------------------

Results[edit]

File:Sam060910-Ecoli sorting-new.jpg

Discussion[edit]