Song Chen:Daily/NOTES/2013-10-16
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FACS sorting[edit]
- Place:UCSD hESC Core
- Type: Sorting(BDInflux, FACSAria 2); Analysis(BDLSRFortessa, FACSCanto 2)
- Brief procedure:
- Dissociate culture into single cells
- Wash twice with PBS
- Filter cells with cell strainer
- Suspend cells best in the density of 106/ml
- Snap cap of FACS tube to avoid contamination
- Place cell suspension in ice (3rd floor)
- Bring culture medium to collect sorted cells