Song Chen:Daily/NOTES/2013-10-16

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FACS sorting[edit]

  • Place:UCSD hESC Core
  • Type: Sorting(BDInflux, FACSAria 2); Analysis(BDLSRFortessa, FACSCanto 2)
  • Brief procedure:
  1. Dissociate culture into single cells
  2. Wash twice with PBS
  3. Filter cells with cell strainer
  4. Suspend cells best in the density of 106/ml
  5. Snap cap of FACS tube to avoid contamination
  6. Place cell suspension in ice (3rd floor)
  7. Bring culture medium to collect sorted cells


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